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ID 34112
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Author
Taguchi, Fumiko
Ogawa, Yujiro
Takeuchi, Kasumi
Suzuki, Tomoko
Abstract
Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 deletion mutant (Delta orf3 mutant) had reduced virulence on tobacco plants. Orf3 shows significant homology to a 3-oxoacyl-(acyl carrier protein) synthase III in the fatty acid elongation cycle. The Delta orf3 mutant had a significantly reduced ability to form acyl homoserine lactones (AHLs), which are quorum-sensing molecules, suggesting that Orf3 is required for AHL synthesis. In comparison with the wild-type strain, swarming motility, biosurfactant production, and tolerance to H2O2 and antibiotics were enhanced in the Delta orf3 mutant. A scanning electron micrograph of inoculated bacteria on the tobacco leaf surface revealed that there is little extracellular polymeric substance matrix surrounding the cells in the Delta orf3 mutant. The phenotypes of the Delta orf3 mutant and an AHL synthesis (Delta psyI) mutant were similar, although the mutant-specific characteristics were more extreme in the Delta orf3 mutant. The swarming motility of the Delta orf3 mutant was greater than that of the Delta psyI mutant. This was attributed to the synergistic effects of the overproduction of biosurfactants and/or alternative fatty acid metabolism in the Delta orf3 mutant. Furthermore, the amounts of iron and biosurfactant seem to be involved in biofilm development under quorum-sensing regulation in P. syringae pv. tabaci 6605.
Keywords
TO-CELL SIGNALS
AERUGINOSA
FLAGELLIN
BIOFILMS
MOTILITY
IRON
IDENTIFICATION
SIDEROPHORES
SPECIFICITY
FLUORESCENT
Note
Digital Object Identifer:10.1128/JB.00763-06
Published with permission from the copyright holder. This is the author's copy, as published in the Journal of Bacteriology, Dec 2006, Volume 188, Issue 24, Pages 8376-8384.
Publisher URL:http://dx.doi.org/10.1128/JB.00763-06
Direct access to Thomson Web of Science record
Copyright © 2006 American Society for Microbiology
Published Date
2006-12
Publication Title
Journal of Bacteriology
Volume
volume188
Issue
issue24
Publisher
American Society for Microbiology
Start Page
8376
End Page
8384
ISSN
0021-9193
NCID
AA0069403X
Content Type
Journal Article
language
英語
Copyright Holders
American Society for Microbiology
File Version
author
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
microbiology_and_immunology/7