このエントリーをはてなブックマークに追加
ID 59964
FullText URL
Author
Du, Juan Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Seno, Akimasa Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama Universityalth Systems ORCID Kaken ID researchmap
Sasada, Saki Graduate School of Natural Science and Technology, Okayama University
Xu, Yanning Department of Pathology, Tianjin Central Hospital of Gynecology Obstetrics
Oo, Aung Ko Ko Graduate School of Natural Science and Technology, Okayama University
Hassan, Ghmkin Graduate School of Natural Science and Technology, Okayama University ORCID
Ueno, Shunsuke Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Afify, Said M. Graduate School of Natural Science and Technology, Okayama University ORCID
Zahra, Maram H Graduate School of Natural Science and Technology, Okayama University
Okada, Nobuhiro Graduate School of Natural Science and Technology, Okayama University Kaken ID researchmap
Chen, Ling Department of Pathology, Tianjin Central Hospital of Gynecology Obstetrics
Fu, Xiaoying School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine
Tokutaka, Heizo SOM Japan
Yan, Ting The Hong Kong University of Science and Technology Medical Center, Shenzhen Peking University
Seno, Masaharu Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Abstract
Background: Cancer stem cells (CSCs) as a class of malignant cancer cells play an important role in tumor progression. Previous studies by our group have demonstrated the establishment of the model of CSCs converting mouse iPS cells (miPSCs) into CSCs by treating the miPSCs with a conditioned medium (CM) of Lewis Lung Carcinoma (LLC) cells with or without the nonmutagenic chemical compounds. CSCs converted from miPSCs developed highly malignant adenocarcinoma when subcutaneously transplanted into the nude mice.
Methods: The miPSCs were treated with each compound for 1 week in the presence of a CM of LLC cells. We evaluated the gene expression in the resultant CSCs comparing that in miPSCs by microarray analysis. And the expression of chemokine (C-C motif) ligand 20 (CCL20) and C-C chemokine receptor type 6 (CCR6) in converted cells were evaluated by rt-qPCR. The CCR6 expression in converted cells and primary cells were determined by flow cytometry.
Results: As the result, the expression of CCL20 was found upregulated in the presence of CM supplemented with PD0325901. Then we assessed the expression of CCR6, which was considered to be stimulated by CCL20. Then the expression of CCR6 was also found up-regulated. Interestingly, IL17A expression was also observed only in the CSCs from the primary tumor implying the effect of tumor microenvironment. Moreover, significantly high level of CCR6 was showed in flow cytometric analysis.
Conclusion: These results suggest that a model of CSCs with CCL20-CCR6 autocrine loop was obtained as the result of the conversion of iPSCs. This CSC should be a good model to study targeting CCR6 as a G protein-coupled receptor (GPCR).
Keywords
miPSCs
CSCs
CCR6
CCL20
Published Date
2020
Publication Title
Journal of Research in Medical and Dental Science
Volume
volume8
Issue
issue1
Publisher
Amber Publication
Start Page
200
End Page
207
ISSN
2347-2367
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
publisher
Web of Science KeyUT
Related Url
isVersionOf https://www.jrmds.in/abstract/upregulated-ccl20-and-ccr6-in-cancer-stem-cells-converted-from-mouse-ips-cells-52887.html
License
https://creativecommons.org/licenses/by-nc/4.0/
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
JP25242045
JP26640079
JP18K-15243