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ID 57849
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Wei, Heng Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Wang, Chen Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Guo, Rui Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Takahashi, Ken Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Naruse, Keiji Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Abstract
Ischemic heart disease remains the largest cause of death worldwide. Accordingly, many researchers have sought curative options, often using laboratory animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart. In this study, we developed a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs). After optimizing the conditions of ischemia, including the concentration of oxygen and duration of application, we evaluated the consequent damage to hiPS-CMs. Notably, exposure to 2% oxygen, 0 mg/ml glucose, and 0% fetal bovine serum increased the percentage of nuclei stained with propidium iodide, an indicator of membrane damage, and decreased cellular viability. These conditions also decreased the contractility of hiPS-CMs. Furthermore, ischemic conditioning increased the mRNA expression of IL-8, consistent with observed conditions in the in vivo heart. Taken together, these findings suggest that our hiPS-CM-based model can provide a useful platform for human ischemic heart disease research.
Keywords
Cardiomyocytes
Human induced pluripotent stem cells
Ischemic heart disease
Myocardial infarction
Published Date
2019-12-10
Publication Title
Biochemical and Biophysical Research Communications
Volume
volume520
Issue
issue3
Publisher
Academic Press
Start Page
600
End Page
605
ISSN
0006291X
NCID
AA00564395
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2019 The Authors.
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Related Url
isVersionOf https://doi.org/10.1016/j.bbrc.2019.09.119
License
http://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Heng Wei, Chen Wang, Rui Guo, Ken Takahashi, Keiji Naruse, Development of a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells, Biochemical and Biophysical Research Communications, Volume 520, Issue 3, 2019, Pages 600-605, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2019.09.119.
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OA
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Non-OpenArchive