Molecular Characterization of High-Level-Cholera-Toxin-Producing El Tor Variant Vibrio cholerae Strains in the Zanzibar Archipelago of Tanzania

Naha, A.; Chowdhury, G.; Ghosh-Banerjee, J.; Senoh, M.; Takahashi, T.; Ley, B.; Thriemer, K.; Deen, J.; Seidlein, L. V.; Ali, S. M.; Khatib, A.; Ramamurthy, T.; Nandy, R. K.; Nair, G. B.; Takeda, Y.; Mukhopadhyay, A. K.

doi:10.1128/JCM.03162-12

Permalink : http://ousar.lib.okayama-u.ac.jp/56700

ID	56700
FullText URL	J_ClinMicrobiol_51_3_1040.pdf 670 KB
Author	Naha, A. 1National Institute of Cholera and Enteric Diseases Chowdhury, G. 1National Institute of Cholera and Enteric Diseases Ghosh-Banerjee, J. 1National Institute of Cholera and Enteric Diseases Senoh, M. Collaborative Research Center of Okayama University for Infectious Diseases at NICED, Takahashi, T. Collaborative Research Center of Okayama University for Infectious Diseases at NICED, Ley, B. The International Vaccine Institute Thriemer, K. The International Vaccine Institute Deen, J. The International Vaccine Institute Seidlein, L. V. Menzies School of Health Research Ali, S. M. Ministry of Health and Social Welfare Khatib, A. Ministry of Health and Social Welfare Ramamurthy, T. 1National Institute of Cholera and Enteric Diseases Nandy, R. K. 1National Institute of Cholera and Enteric Diseases Nair, G. B. 1National Institute of Cholera and Enteric Diseases Takeda, Y. Collaborative Research Center of Okayama University for Infectious Diseases at NICED, Mukhopadhyay, A. K. 1National Institute of Cholera and Enteric Diseases
Abstract	Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis.
Keywords	Vibrio cholerae ctxB Cholera PFGE
Note	This is an Accepted Manuscript of an article published by American Society for Microbiology
Published Date	2013-03
Publication Title	Journal of Clinical Microbiology
Volume	volume51
Issue	issue3
Publisher	American Society for Microbiology
Start Page	1040
End Page	1045
ISSN	00951137
NCID	AA00695531
Content Type	Journal Article
language	英語
OAI-PMH Set	岡山大学
File Version	author
PubMed ID	23325815
DOI	10.1128/JCM.03162-12
Web of Science KeyUT	000315121700055
Related Url	isVersionOf https://doi.org/10.1128/JCM.03162-12
Project	Collaborative Research of Okayama University for Infectious Diseases in India