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ID 56686
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Abdel‐Sattar, El‐Shaymaa Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Miyoshi, Shin‐ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Elgaml, Abdelaziz Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Abstract
Vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. This pathogen secretes a relatively important toxin named V. mimicus metalloprotease (VMP). In this study, we clarified regulation of the VMP production according to the quorum-sensing master regulatory protein named LuxR. First, the full length of luxR gene, encoding LuxR, was detected in V. mimicus strain E-37, an environmental isolate. Next, the putative consensus binding sequence of LuxR protein could be detected in the upstream (promoter) region of VMP encoding gene, vmp. Finally, the effect of disruption of luxR gene on the expression of vmp and production of VMP was evaluated. Namely, the expression of vmp was significantly diminished by luxR disruption and the production of VMP was severely altered. Taken together, here we report that VMP production is under the positive regulation of the quorum-sensing master regulatory protein, LuxR.
Keywords
LuxR protein
Metalloprotease
Quorum-sensing
Vibrio mimicus
Note
This is an Accepted Manuscript of an article published by WILEY-BLACKWELL
Published Date
2016-05-10
Publication Title
Journal of Basic Microbiology
Volume
volume56
Issue
issue10
Publisher
WILEY-BLACKWELL
Start Page
1051
End Page
1058
ISSN
0233111X
NCID
AA10522008
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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Related Url
isVersionOf https://doi.org/10.1002/jobm.201600002
Project
Collaborative Research of Okayama University for Infectious Diseases in India