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ID 50622
FullText URL
Author
Jiyou, Wang
Katoh, Keizo
Senoh, Mitsutoshi
Maehara, Yoko
Abstract
Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.
Keywords
Polymerase chain reaction
Purification
Serine protease
Metalloprotease
Vibrio vulnificus
Published Date
2012-04
Publication Title
World Journal of Microbiology & Biotechnology
Volume
volume28
Issue
issue4
Publisher
Springer
Start Page
1633
End Page
1639
ISSN
0959-3993
Content Type
Journal Article
Project
Collaborative Research of Okayama University for Infectious Diseases in India
Official Url
http://dx.doi.org/10.1007/s11274-011-0969-y
language
英語
File Version
author
Refereed
True
DOI
Web of Science KeyUT