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ID 48979
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Author
Takenami, Mami
Kuroda, Yuka
Hyakutake, Toru
Yanase, Shinichiro Kaken ID publons researchmap
Abstract
The microfluidic sperm-sorting (MFSS) device is a promising advancement for assisted reproductive technology. Previously, poly(dimethylsiloxiane) and quartz MFSS devices were developed and used for intracytoplasmic sperm injection. However, these disposable devices were not clinically suitable for assisted reproduction, so a cyclo-olefin polymer MFSS (COP-MFSS) device was developed. By micromachining, two microfluidic channels with different heights and widths (chip A: 0.3 x 0.5 mm; chip B: 0.1 x 0.6 mm) were prepared. Sorted sperm concentrations were similar in both microfluidic channels. Linear-velocity distribution using the microfluidic channel of chip B was higher than that of chip A. Using confocal fluorescence microscopy, it was found that the highest number of motile spermatozoa swam across the laminar flow at the bottom of the microfluidic channel. The time required to swim across the laminar flow was longer at the bottom and top of the microfluidic channels than in the middle because of the low fluid velocity. These results experimentally demonstrated that the width of microfluidic channels should be increased in the region of laminar flow from the semen inlet to the outlet for unsorted spermatozoa to selectively recover spermatozoa with high linear velocity.
Keywords
laminar flow
linear velocity
microfluidic sperm sorting
motility
Published Date
2012-01
Publication Title
Reproductive BioMedicine Online
Volume
volume24
Issue
issue1
Publisher
Elsevier
Start Page
109
End Page
115
ISSN
1472-6483
Content Type
Journal Article
Project
Research Core for Interdisciplinary Sciences
Official Url
http://www.sciencedirect.com/science/article/pii/S1472648311004937
language
英語
Copyright Holders
Copyright © 2011 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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