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ID 57903
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Sakakibara, Akira Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID researchmap
Takebe, So Graduate School of Biology-Oriented Science and Technology, Kindai University
Ide, Toru Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hayakawa, Tohru Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Abstract
Cry46Ab from Bacillus thuringiensis TK-E6 is a new mosquitocidal toxin with aerolysin-type architecture, and has been shown that co-administration of Cry46Ab with other mosquitocidal Cry toxins results in synergistic toxicity against Culex pipiens Coquillett (Diptera: Culicidae) mosquito larvae. Cry46Ab, therefore, is expected to find use in improving the insecticidal activity of B. thuringiensis-based bioinsecticides. In the present study, the mode of action of Cry46Ab was explored by single-channel measurements of Cry46Ab channel-pores. The single-channel conductances of channel-pores formed in planar lipid bilayers by Cry46Ab were determined to be 31.8 +/- 2.7 pS in 150 mM NaCl and 24.2 +/- 0.7 pS in 150 mM CaCl2. Ion-selectivity measurements revealed that the channel-pores formed by Cry46Ab were cation selective. The permeability ratio of K+ to Cl-was approximately 4, and the preferences for cations were K+ > Na+, K+ > Ca2+, and Ca2+ > Na+. A calcein release assay using liposomes suggested that Cry46Ab influences the integrity of membrane vesicles. Formation of cation-selective channel-pores has been observed with other insecticidal Cry toxins that have structures distinct from those of Cry46Ab; the capability of forming such pores may be a property required of insecticidal toxins.
Keywords
Bacillus thuringiensis
Cry46Ab toxin
Planar lipid bilayer
Single-channel analysis
Calcein release assay
Published Date
2019-08-16
Publication Title
Applied Entomology and Zoology
Volume
volume54
Issue
issue4
Publisher
Springer
Start Page
389
End Page
398
ISSN
0003-6862
NCID
AA00543238
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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author
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1007/s13355-019-00635-z
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Sakakibara, A., Takebe, S., Ide, T. et al. Characterization of the channel-pores formed by Bacillus thuringiensis Cry46Ab toxin in planar lipid bilayers. Appl Entomol Zool 54, 389–398 (2019). https://doi.org/10.1007/s13355-019-00635-z
Funder Name
Japan Society for the Promotion of Science
助成番号
JP18K05675