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ID 51875
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Author
Kikuchi, Takeshi
Asaumi, Koji
Kawaki, Harumi
Nishida, Takashi Kaken ID researchmap
Kawata, Kazumi
Mitani, Shigeru
Tabata, Yasuhiko
Takigawa, Masaharu Kaken ID publons researchmap
Abstract
CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique molecule that promotes the entire endochondral ossification process and regeneration of damaged articular cartilage. Also, CCN2 has been shown to enhance the adhesion and migration of bone marrow stromal cells as well as the growth and differentiation of osteoblasts; hence, its utility in bone regeneration has been suggested. Here, we evaluated the effect of CCN2 on the regeneration of an intractable bone defect in a rat model. First, we prepared two recombinant CCN2s of different origins, and the one showing the stronger effect on osteoblasts in vitro was selected for further evaluation, based on the result of an in vitro bioassay. Next, to obtain a sustained effect, the recombinant CCN2 was incorporated into gelatin hydrogel that enabled the gradual release of the factor. Evaluation in vivo indicated that CCN2 continued to be released at least for up to 14 days after its incorporation. Application of the gelatin hydrogel-CCN2 complex, together with a collagen scaffold to the bone defect prepared in a rat femur resulted in remarkable induction of osteoblastic mineralization markers within 2 weeks. Finally, distinct enhancement of bone regeneration was observed 3 weeks after the application of the complex. These results confirm the utility of CCN2 in the regeneration of intractable bone defects in vivo when the factor is incorporated into gelatin hydrogel.
Published Date
2008-06
Publication Title
Tissue Engineering Part A
Volume
volume14
Issue
issue6
Publisher
Mary Ann Liebert, Inc.
Start Page
1089
End Page
1098
ISSN
1937-3341
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1089/ten.tea.2007.0167
language
英語
Copyright Holders
© Mary Ann Liebert, Inc.
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publisher
Refereed
True
DOI
PubMed ID
Web of Science KeyUT