start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue= article-no= start-page=1673581 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2026 dt-pub=20260107 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Binding of IgA1 and surface-expressed collagen-binding protein of Streptococcus mutans contributes to IgA nephropathy pathogenesis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background: The present study was conducted to examine the interaction between collagen-binding protein (Cnm) of Streptococcus mutans and immunoglobulin (IgA) to clarify the possible involvement in IgA nephropathy (IgAN) development.
Methods: The binding of Cnm to human immunoglobulins was examined using an enzyme-linked immunosorbent assay. A nephritis-induced rat model was employed to confirm the localization of Cnm.
Results: IgA1 showed significantly greater binding ability to Cnm than to other bacterial surface proteins, and Cnm showed significantly greater binding ability to IgA1 than to other immunoglobulins. In rats administered Cnm, IgA deposition was observed in the glomerular mesangial region. Furthermore, biotin-labeled Cnm was observed in the same region as IgA deposition in the Cnm group.
Conclusions: Taken together, it is considered that following invasion into the bloodstream, Cnm binds to and forms a complex with IgA1, leading to deposition of IgA1 in renal glomeruli. en-copyright= kn-copyright= en-aut-name=MatsuokaDaiki en-aut-sei=Matsuoka en-aut-mei=Daiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SueharaKana en-aut-sei=Suehara en-aut-mei=Kana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakaShuhei en-aut-sei=Naka en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MisakiTaro en-aut-sei=Misaki en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NagasawaYasuyuki en-aut-sei=Nagasawa en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ItoSeigo en-aut-sei=Ito en-aut-mei=Seigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SuehiroYuto en-aut-sei=Suehiro en-aut-mei=Yuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NomuraRyota en-aut-sei=Nomura en-aut-mei=Ryota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NakanoKazuhiko en-aut-sei=Nakano en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=Matsumoto-NakanoMichiyo en-aut-sei=Matsumoto-Nakano en-aut-mei=Michiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Division of Nephrology, Seirei Hamamatsu General Hospital kn-affil= affil-num=5 en-affil=Department of General Internal Medicine, Hyogo Medical University kn-affil= affil-num=6 en-affil=Department of Internal Medicine, Japan Self-Defense Force Iruma Hospital kn-affil= affil-num=7 en-affil=Department of Pediatric Dentistry, Graduate School of Dentistry, The University of Osaka kn-affil= affil-num=8 en-affil=Department of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima University kn-affil= affil-num=9 en-affil=Department of Pediatric Dentistry, Graduate School of Dentistry, The University of Osaka kn-affil= affil-num=10 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=bacterial surface proteins kn-keyword=bacterial surface proteins en-keyword=collagen-binding protein kn-keyword=collagen-binding protein en-keyword=human immunoglobulins kn-keyword=human immunoglobulins en-keyword=IgA nephropathy kn-keyword=IgA nephropathy en-keyword=Streptococcus mutans kn-keyword=Streptococcus mutans END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=994014 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220913 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability en-subtitle= kn-subtitle= en-abstract= kn-abstract=Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries. The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown. In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm. Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure. Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated. The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides. Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains. Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability. en-copyright= kn-copyright= en-aut-name=NakaShuhei en-aut-sei=Naka en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuokaDaiki en-aut-sei=Matsuoka en-aut-mei=Daiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=GotoKana en-aut-sei=Goto en-aut-mei=Kana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MisakiTaro en-aut-sei=Misaki en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NagasawaYasuyuki en-aut-sei=Nagasawa en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ItoSeigo en-aut-sei=Ito en-aut-mei=Seigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NomuraRyota en-aut-sei=Nomura en-aut-mei=Ryota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakanoKazuhiko en-aut-sei=Nakano en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=Matsumoto-NakanoMichiyo en-aut-sei=Matsumoto-Nakano en-aut-mei=Michiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Division of Nephrology, Seirei Hamamatsu General Hospital kn-affil= affil-num=5 en-affil=Department of General Internal Medicine, Hyogo College of Medicine kn-affil= affil-num=6 en-affil=Department of Internal Medicine, Japan Self-Defense Iruma Hospital kn-affil= affil-num=7 en-affil=Department of Pediatric Dentistry, Division of Oral infection and Disease Control, Osaka University Graduate School of Dentistry kn-affil= affil-num=8 en-affil=Department of Pediatric Dentistry, Division of Oral infection and Disease Control, Osaka University Graduate School of Dentistry kn-affil= affil-num=9 en-affil=Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Streptococcus mutans kn-keyword=Streptococcus mutans en-keyword=collagen-binding protein kn-keyword=collagen-binding protein en-keyword=membrane permeability kn-keyword=membrane permeability en-keyword=cell structure kn-keyword=cell structure en-keyword=RNA-seq kn-keyword=RNA-seq END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=4 article-no= start-page=1086 end-page=1089 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Three antinematodal diterpenes from Euphorbia kansui en-subtitle= kn-subtitle= en-abstract= kn-abstract=Three compounds, 20-O-acetyl-[3-O-(2'E,4'Z)-deca-dienoyl]-ingenol (1), 20-O-acetyl-[5-O-(2'E,4'Z)-decadienoyl]-ingenol (2) and 3-O-(2'E,4'Z)-decadienoylingenol (3), were isolated from Euphorbia kansui under the bioassay-guided method. Each compound showed the same antinematodal activity against the nematode, Bursaphelenchus xylophilus, at a minimum effective dose (MED) of 5 mu g/cotton ball. en-copyright= kn-copyright= en-aut-name=ShiJian-Xiao en-aut-sei=Shi en-aut-mei=Jian-Xiao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=LiZhi-Xuan en-aut-sei=Li en-aut-mei=Zhi-Xuan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NitodaTeruhiko en-aut-sei=Nitoda en-aut-mei=Teruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IzumiMinoru en-aut-sei=Izumi en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=College of Life Science, Northwest University affil-num=2 en-affil= kn-affil=College of Life Science, Northwest University affil-num=3 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=6 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=7 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University affil-num=8 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Department of Biofunctional Chemistry, Laboratory of Applied Natural Products Chemistry, Okayama University en-keyword=antinematodal activity kn-keyword=antinematodal activity en-keyword=Bursaphelenchus xylophilus kn-keyword=Bursaphelenchus xylophilus en-keyword=diterpenoid kn-keyword=diterpenoid en-keyword=ingenane kn-keyword=ingenane en-keyword=Euphorbia kansui kn-keyword=Euphorbia kansui END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=3 article-no= start-page=826 end-page=829 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Glucosylation of Sucrose Laurate with Cyclodextrin Glucanotransferase en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sucrose monolauroyl esters were found to serve as substrates for cyclodextrin glucanotransferase (CGTase)-catalyzed transglucosidation reactions, affording new sucrose esters that have an additional 1-3 glucose residues on the pyranose ring of the sucrose moiety in the ester. en-copyright= kn-copyright= en-aut-name=OkadaKatsuhide en-aut-sei=Okada en-aut-mei=Katsuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ZhaoHaisuo en-aut-sei=Zhao en-aut-mei=Haisuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IzumiMinoru en-aut-sei=Izumi en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Research & Development Center, Hayashibara Biochemical Laboratories, Inc. affil-num=2 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University en-keyword=cyclodextrin glucanotransferase kn-keyword=cyclodextrin glucanotransferase en-keyword=CGTase kn-keyword=CGTase en-keyword=sucrose monolaurate kn-keyword=sucrose monolaurate en-keyword=surfactant kn-keyword=surfactant END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=4 article-no= start-page=1078 end-page=1082 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of lipid derivatives of pyrrole polyamide and their biological activity en-subtitle= kn-subtitle= en-abstract= kn-abstract=Novel fatty acyl and phospholipid derivatives of pyrrole polyamide were synthesized. Their cytotoxicity against a cancer cell line of MT-4 cells and those infected by human immunodeficiency virus (HIV) was examined. Although no anti-HIV activity was found, their cytotoxicitty against the cancer cells was significantly enhanced by introducing a lipophilic group into the pyrrole polyamide. en-copyright= kn-copyright= en-aut-name=YamamotoMasahiko en-aut-sei=Yamamoto en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ZhuChangjin en-aut-sei=Zhu en-aut-mei=Changjin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YiLui en-aut-sei=Yi en-aut-mei=Lui kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=RongZheng en-aut-sei=Rong en-aut-mei=Zheng kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiuraYoshie en-aut-sei=Miura en-aut-mei=Yoshie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IzumiMinoru en-aut-sei=Izumi en-aut-mei=Minoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TanamotoKen-ichi en-aut-sei=Tanamoto en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ShimizuSakayu en-aut-sei=Shimizu en-aut-mei=Sakayu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=School of Chemical Engineering & Environment, Beijing Institute of Technology affil-num=3 en-affil= kn-affil=School of Chemical Engineering & Environment, Beijing Institute of Technology affil-num=4 en-affil= kn-affil=School of Chemical Engineering & Environment, Beijing Institute of Technology affil-num=5 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=National Institute of Health Sciences, Division of Food Additives affil-num=9 en-affil= kn-affil=Department of Applied Bioscience, Graduate School of Agriculture, Kyoto University affil-num=10 en-affil= kn-affil=Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=pyrrole polyamide kn-keyword=pyrrole polyamide en-keyword=lipid kn-keyword=lipid en-keyword=phospholipid kn-keyword=phospholipid en-keyword=cancer cell kn-keyword=cancer cell en-keyword=human immunodeficiency virus (HIV)-II kn-keyword=human immunodeficiency virus (HIV)-II END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=71 end-page=84 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=リン脂質過酸化物の生物有機化学的合成 kn-title=Chemoenzymatic Synthesis of Phospholipid Hydroperoxides en-subtitle= kn-subtitle= en-abstract=健康正常人の血液などの組織中にはリン脂質過酸化物が極微量で存在し，疾病や老化によってその濃度が顕著に上昇する事が知られている．その事が明らかにされた当初は，極めて複雑な混合物をなす生体脂質中に含まれる不安定な極微量の過酸化脂質を単離・構造決定する事は殆ど不可能と考えられていた．現在もなお，そのような脂質過酸化物を生体組織から純粋に取り出し，構造決定したという報告は無い．従ってそのような分子種の化学的・生理学的性質は不明であったが，脂肪酸過酸化物が毒性を示す事から，リン脂質過酸化物もおそらく毒性を示すだろうと考えられてきた．このような漠然とした推定を科学的に明らかにするためには，化学合成によらざるを得ない．我々はこの未知の合成に取りかかった．しかし，従来の化学的手法のみでは不可能である事も明らかであった．その中で予想された困難の一つは極めて不安定なヒドロペルオキシ基を不飽和脂肪酸のある特定の位置にどのように導入するかという問題と，ヒドロペルオキシ基に影響を与える事なく合成中間体をどのように化学変換するかであった．第一の問題に対する解決策として，不飽和脂肪酸に大豆リポキシゲナーゼを作用させる事で解決する事ができた．植物に広く分布する酵素であるリポキシゲナーゼは植物中でリノール酸に作用して過酸化し，その生成物にヒドロペルオキシドリアーゼという酵素が作用して種々のアルデヒドが精製し，これは植物の青臭みを与える．第二の問題に対しては，リノール酸に導入された不安定なヒドロペルオキシ基をパーアセタールとしての保護する事により解決した．この保護基は，中間体から最終生成物に至るまでの反応条件，例えばDCCによるアシル化反応に対して安定である事が明らかとなった．この二つの問題を解決する事によって，リン脂質過酸化物の一つであるホスファチジルコリン過酸化物を世界に先駆けて成功した．さらにこのホスファチジルコリン過酸化物に微生物由来のホスフォリパーゼDを作用させる事によってホスファチジルエタノールアミン過酸化物，ホスファチジルセリン過酸化物やホスファチジルグリセロール過酸化物の合成にも成功した．また，トリグリセリド過酸化物の合成も可能になった．これらの脂質過酸化物が化学的に実態のあるものとして認識されてから，その生理作用に関する研究が広範に行われている．しかし，生体組織に存在するリン脂質過酸化物の生理学的役割は依然として明らかになっていない．ある種のリン脂質過酸化物が動物の免疫系を活性化するという報告もあり，必ずしも生体に対して悪い作用をするばかりではないようである． kn-abstract=Chemoenzymatic synthesis of 1-stearoyl-2-hydropeoxyacyl-sn-glycerophospholipids including phosphatidylcholine (PC-OOH), phosphatidic acid (PA-OOH), phosphatidylethanolamine (PE-OOH), phosphatidylglycerol (PG-OOH) and phosphatidylserine (PS-OOH). The hydroperoxy acyl moieties were prepared via hydroperoxidation of linoleic, dihomo-γ-linolenic and arachidonic acids by soybean, potate lipoxygenase or autoxidation. Their hydroperoxy group was protected as a dimethylperacetal before condensation with lysophosphatidylcholine. Optically active lysophosphatidylcholine was prepared via short pathway involving lipase-catalyzed direct enantioselective stearoylation of 2-O-benzylglycerol and choline phosphate synthesis. Peroxy fatty acids and lysophosphatidylcholine thus obtained were condensed using dicyclohexylcarbodiimide in chloroform. Removing the peracetal group in the product and purification by reverse-phase chromatography afforded the desired PC-OOH’s. PA-OOH, PG-OOH, PE-OOH and PS-OOH were obtained by phospholipase-D catalyzed transphosphatidylation from PC-OOH. As a reference compound for biological studies of hydroperoxy phopholipid, PC-OH's were also prepared in which hydroxy unsaturated fatty acyl group was linked to the sn-2 position of the glycerophospholipids. en-copyright= kn-copyright= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=1 ORCID= en-aut-name=YonedaKenji en-aut-sei=Yoneda en-aut-mei=Kenji kn-aut-name=米田健司 kn-aut-sei=米田 kn-aut-mei=健司 aut-affil-num=2 ORCID= en-aut-name=SasakuraKeiji en-aut-sei=Sasakura en-aut-mei=Keiji kn-aut-name=笹倉敬司 kn-aut-sei=笹倉 kn-aut-mei=敬司 aut-affil-num=3 ORCID= en-aut-name=ShigetaYasutami en-aut-sei=Shigeta en-aut-mei=Yasutami kn-aut-name=繁田泰民 kn-aut-sei=繁田 kn-aut-mei=泰民 aut-affil-num=4 ORCID= en-aut-name=KishidaYasuhiro en-aut-sei=Kishida en-aut-mei=Yasuhiro kn-aut-name=岸田靖弘 kn-aut-sei=岸田 kn-aut-mei=靖弘 aut-affil-num=5 ORCID= en-aut-name=AoishiAkihiro en-aut-sei=Aoishi en-aut-mei=Akihiro kn-aut-name=青石晃宏 kn-aut-sei=青石 kn-aut-mei=晃宏 aut-affil-num=6 ORCID= en-aut-name=DaidoHiroko en-aut-sei=Daido en-aut-mei=Hiroko kn-aut-name=大同浩子 kn-aut-sei=大同 kn-aut-mei=浩子 aut-affil-num=7 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=8 ORCID= en-aut-name=IwasaJunkichi en-aut-sei=Iwasa en-aut-mei=Junkichi kn-aut-name=岩佐順吉 kn-aut-sei=岩佐 kn-aut-mei=順吉 aut-affil-num=9 ORCID= en-aut-name=TaharaShoichi en-aut-sei=Tahara en-aut-mei=Shoichi kn-aut-name=田原正一 kn-aut-sei=田原 kn-aut-mei=正一 aut-affil-num=10 ORCID= en-aut-name=KanekoTakao en-aut-sei=Kaneko en-aut-mei=Takao kn-aut-name=金子孝夫 kn-aut-sei=金子 kn-aut-mei=孝夫 aut-affil-num=11 ORCID= en-aut-name=MatsuoMitsuyoshi en-aut-sei=Matsuo en-aut-mei=Mitsuyoshi kn-aut-name=松尾光芳 kn-aut-sei=松尾 kn-aut-mei=光芳 aut-affil-num=12 ORCID= en-aut-name=ShimizuSakayu en-aut-sei=Shimizu en-aut-mei=Sakayu kn-aut-name=清水昌 kn-aut-sei=清水 kn-aut-mei=昌 aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=岡山大学 affil-num=8 en-affil= kn-affil=岡山大学 affil-num=9 en-affil= kn-affil=岡山大学 affil-num=10 en-affil= kn-affil=東京都老人総合研究所 affil-num=11 en-affil= kn-affil=東京都老人総合研究所 affil-num=12 en-affil= kn-affil=東京都老人総合研究所 affil-num=13 en-affil= kn-affil=京都大学農学部 en-keyword=phospholipid kn-keyword=phospholipid en-keyword=peroxide kn-keyword=peroxide en-keyword=hydroperoxide kn-keyword=hydroperoxide en-keyword=phospholipase D kn-keyword=phospholipase D en-keyword=lipoxygenase kn-keyword=lipoxygenase END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=1 article-no= start-page=7 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19950201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ケニヤ産植物、Psiadia punctulata に含まれる植物生長調節物質 kn-title=Plant Growth Regulators from Kenyan Plant, Psiadia punctulata en-subtitle= kn-subtitle= en-abstract=植物の生長を制御する物質を広く天然に求めることは、より安全で有効な農薬およびその関連物質を開発するための重要な課題である。本研究では、レタス幼苗の生長に対する調節物質の探索をケニヤ産植物Psiadia punctulata について行った。その結果、同植物のメタノール抽出物より、5-hydroxy-7,2',3',4',5'-pentamethoxyflavone 及び　5,3'-dihydroxy-7,2',4',5'-tetramethoxyflavone を調整物質として単離した。前者は、根や胚軸の長さを130％まで増加させたが、全体の重量はほぼ80％にまで減少させた。一方、後者も同様の活性パターンを示し、根を140％伸張させ植物体重量を58％まで減少させるという興味ある現象が観察された。　 kn-abstract=Compounds having plant growth regulatory effect were found in Kenyan Psiadia punctulata, and two flavones,5-hydroxy-7,2',3',4',5'-pentamethoxyflavone(1) and 5,3'-dihydroxy-7,2',4',5'-tetramethoxyflavone(2) were isolated and identified as the activ constituents. en-copyright= kn-copyright= en-aut-name=KerikoJoseph Mungai en-aut-sei=Keriko en-aut-mei=Joseph Mungai kn-aut-name=ケリコジョセフ ムンガイ kn-aut-sei=ケリコ kn-aut-mei=ジョセフ ムンガイ aut-affil-num=1 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=2 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=3 ORCID= en-aut-name=IsozakiYumika en-aut-sei=Isozaki en-aut-mei=Yumika kn-aut-name=磯崎友実加 kn-aut-sei=磯崎 kn-aut-mei=友実加 aut-affil-num=4 ORCID= en-aut-name=IwasaJunkichi en-aut-sei=Iwasa en-aut-mei=Junkichi kn-aut-name=岩佐順吉 kn-aut-sei=岩佐 kn-aut-mei=順吉 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Kenyan plant kn-keyword=Kenyan plant en-keyword=Psiadia punctulata(Asteraceae) kn-keyword=Psiadia punctulata(Asteraceae) en-keyword=flavonoids kn-keyword=flavonoids en-keyword=growth regulator kn-keyword=growth regulator en-keyword=lettuce seeds kn-keyword=lettuce seeds END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=1 article-no= start-page=21 end-page=25 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=199702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Methyl 13-hydroperoxyoctadecadienoateの分解に対する蔬菜類の抑制効果 kn-title=Preventive Action of Vegetables on Degradation of Methyl 13-Hydroperoxyoctadecadienoate en-subtitle= kn-subtitle= en-abstract=不飽和脂質の酸化防止に関する研究は今日なお活発に行われており、その食品学的重要性と問題の難しさには変わり無い。自動酸化によって生成した過酸化物は極めて容易に分解し、その分解生成物は食品の重大な劣化を招き、健康を害することは良く知られている。本研究ではMethly 13-hydroperoxyoctadecadienoateの分解の抑制について検討したところ、ある種の蔬菜のクロロホルム―メタノール抽出物に分解阻止効果があることが明らかになった。例えばいんげん豆抽出物は過酸化物に対して、60℃、96時間の過熱においても約60%の分解抑制作用を示すことが判明した。 kn-abstract=The preventive actions of some vegetables on the degradation of 13-hydroperocxyoctadecadienoate were investigated.Among these,kindly bean extract prevented the degradation of the hydroperoxide at about 60% followed by green pepper,cucumber,spinach pumpkin,mushroom,carrot,and shungiku to defferent degrees. en-copyright= kn-copyright= en-aut-name=AlamMd Khorshed en-aut-sei=Alam en-aut-mei=Md Khorshed kn-aut-name=アラムモハムド コルシェド kn-aut-sei=アラム kn-aut-mei=モハムド コルシェド aut-affil-num=1 ORCID= en-aut-name=ShimizuRumiko en-aut-sei=Shimizu en-aut-mei=Rumiko kn-aut-name=清水るみ子 kn-aut-sei=清水 kn-aut-mei=るみ子 aut-affil-num=2 ORCID= en-aut-name=YamadaEiko en-aut-sei=Yamada en-aut-mei=Eiko kn-aut-name=山田英子 kn-aut-sei=山田 kn-aut-mei=英子 aut-affil-num=3 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=4 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Methyl 13-hydroperoxy octadecadienoate kn-keyword=Methyl 13-hydroperoxy octadecadienoate en-keyword=Degradation of hydroperoxide kn-keyword=Degradation of hydroperoxide END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=1 article-no= start-page=7 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=199702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ノコギリヒラタムシ食害小麦に含まれる定着活性ケトステロイド kn-title=Ketosteroids As Arrestants to Oryzaephilus surinamensis (L.) from Wheat Flour Infested by the Same Weevil en-subtitle= kn-subtitle= en-abstract=世界的に著名な貯穀害虫であるノコギリヒラタムシによって食害された小麦のヘキサン抽出物中には、未食害の小麦には含まれない、数種のノコギリヒラタムシ定着活性物質が存在し、このうちの2種の活性物質が既に構造解明された。本研究では種々の機器分析、および市販化合物からの誘導などにより、未知の活性物質がcholestan-3-one,ergostan-3-one,stifmastan-3-one の混合物であると同定した。 kn-abstract=From hexane extract of wheat flour infested by the sawtoothed gain beetle [Oryzaephilus surinamensis (L.); Coleoptera; Silvanidae, three ketosteroids,cholestan-3-one(3),ergostan-3-one(4)and stigmastan-3-one(5),were obtained in a mixture and identified as arrestants to this weevil. en-copyright= kn-copyright= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=1 ORCID= en-aut-name=OkamotoAkiko en-aut-sei=Okamoto en-aut-mei=Akiko kn-aut-name=岡村愛子 kn-aut-sei=岡村 kn-aut-mei=愛子 aut-affil-num=2 ORCID= en-aut-name=SugawaraKeiji en-aut-sei=Sugawara en-aut-mei=Keiji kn-aut-name=菅原敬二 kn-aut-sei=菅原 kn-aut-mei=敬二 aut-affil-num=3 ORCID= en-aut-name=TakedaTaro en-aut-sei=Takeda en-aut-mei=Taro kn-aut-name=竹田太郎 kn-aut-sei=竹田 kn-aut-mei=太郎 aut-affil-num=4 ORCID= en-aut-name=IwasaJunkichi en-aut-sei=Iwasa en-aut-mei=Junkichi kn-aut-name=岩佐順吉 kn-aut-sei=岩佐 kn-aut-mei=順吉 aut-affil-num=5 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Oryzaephilus surinamensis(L.) kn-keyword=Oryzaephilus surinamensis(L.) en-keyword=infested wheat flour kn-keyword=infested wheat flour en-keyword=arrestants kn-keyword=arrestants en-keyword=cholestan-3-one kn-keyword=cholestan-3-one en-keyword=ergostan-3-one and stigmastan-3-one kn-keyword=ergostan-3-one and stigmastan-3-one END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=65 end-page=69 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=過酸化脂質に対するリパーゼの触媒活性 kn-title=Lipase Activity for Lipid Hydroperoxides en-subtitle= kn-subtitle= en-abstract=自然界において通常の環境でその役割を果たす酵素の多くは特殊な環境下おいては活性を示さないか失活することが多い。不飽和脂肪酸を結合するトリグリセリドは食品等の劣化にともなって容易に自動酸化をうけて脂質過酸化物を生じ、ヒドロペルオキシドの高い反応性に加えてこれらは周囲に強い酸化的環境を生み出す。本研究ではこのような状況下に基質としての過酸化脂質に対してリパーゼがどのような活性を示すかを検討した。その結果、Pseudomonas fluorescens由来のリパーゼP(Amano)はヒドロペルオキシドのアシル化反応において有機溶媒中で通常と変わらない触媒作用を示し、しかもその反応は立体選択的に進行することが明らかとなった。有機溶媒中、過酸化物の存在という特殊な環境下における上記生体触媒反応は光学活性過酸化物の生産の新しい方法としての可能性を示すものである。一方、水系では過酸化脂質に対して上記リパーゼは正常な触媒作用を示さずリノール酸より合成されたラセミ型過酸化不飽和脂肪酸エステルの過水分解に対して生成物の複雑な混合物を与えた。以上のように特殊な環境下における微生物由来のリパーゼの活性は反応条件の違いにより変化することが本研究で明らかとなった。 kn-abstract=Catalytic activity of lipase P (Amano) from pseudomonas fluorescens for lipid hydroperoxides were examined and in organic solvent the enzyme was found to catalyze acylation reaction of the hydroperoxy group in a stereoselective manner producing optically active hydroperoxy compounds, whereas in aqueous medium the lipase-catalyzed ester hydrolysis afforded a complicated mixture of unknown reaction products. en-copyright= kn-copyright= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=1 ORCID= en-aut-name=HirotaNaohisa en-aut-sei=Hirota en-aut-mei=Naohisa kn-aut-name=廣田尚久 kn-aut-sei=廣田 kn-aut-mei=尚久 aut-affil-num=2 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=lipase kn-keyword=lipase en-keyword=lipid hydroperoxide kn-keyword=lipid hydroperoxide en-keyword=hydroperoxide kn-keyword=hydroperoxide en-keyword=Pseudomonas fluorescens kn-keyword=Pseudomonas fluorescens END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=17 end-page=21 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ケニア産キク科薬用植物Vernonia hindiiに含まれる生物活性物質 kn-title=Biologically Active Substance from Kenyan Plant, Vernonia hindii S. Moore (Asteraceae) en-subtitle= kn-subtitle= en-abstract=熱帯・砂漠など特殊な環境に自生する植物には、生物活性2次代謝物質を含むものが多く、古くから民間伝承薬や天然殺虫剤として使用されてきた。しかし、まだ十分に調査・研究されていない植物も残されている。本研究では、ケニアに自生するキク科薬物植物のVernonia hindiiに含まれる活性物質の単離と構造解明を行い、その生物活性を調べた。植物生長制御試験を指標としてV . hindiiのメタノール抽出物を文画・精製した結果、新規の化学構造をもつ植物生長抑制物質を単離した。さらに、種々の分析機器を用いて化学構造を解析し、stigmastan型のステロイド配糖体であると決定した。この化合物は100μg / discでコントロールと比べ、24％まっでレタスの生長を抑制した。 kn-abstract=A novel stigmastane-type steroid glucoside glucoside has been isolated from the aerial parts of Vernonia hindii.The structure was elucidated by spectroscopic methods.The compound exhibitewd lettuce seedling growth inhibitory activity. en-copyright= kn-copyright= en-aut-name=KenjiGlaston Mwangi en-aut-sei=Kenji en-aut-mei=Glaston Mwangi kn-aut-name=ケンジグラストン ムアンギ kn-aut-sei=ケンジ kn-aut-mei=グラストン ムアンギ aut-affil-num=1 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=2 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=3 ORCID= en-aut-name=IwasaJunkichi en-aut-sei=Iwasa en-aut-mei=Junkichi kn-aut-name=岩佐順吉 kn-aut-sei=岩佐 kn-aut-mei=順吉 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Jomo Kenyatta University of Agriculture and Technology affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=biologically active substance kn-keyword=biologically active substance en-keyword=Kenyan plant kn-keyword=Kenyan plant en-keyword=Vernonia hindii kn-keyword=Vernonia hindii en-keyword=steroid glucoside kn-keyword=steroid glucoside en-keyword=lettuce seedling growth inhibitor kn-keyword=lettuce seedling growth inhibitor END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=23 end-page=27 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Volatile, from Validamycin Producer kn-title=バリダマイシン生産菌が生成する揮発物質 en-subtitle= kn-subtitle= en-abstract=バイダマイシンA（以後VM-Aと略称する）はStreptomyces hygroscopicus subsp. limoneusによって生産されるアミノ酸糖体抗生物資の一つで、Pellicularia saskiiによって惹起されるイネ紋枯病の防除ばかりでなく、そ菜苗立枯病、白絹病などにも防除効果を示す低毒性農薬として広く用いられている。我々はこの抗生物質生産時にその培養液中に芳香性揮発物質が生成されることを見いだした。従来、多くの放線菌代謝産物として土壌臭物質が分離され、ジオスミン、メチルイソボルネオールなどと同定されている。しかし、放線菌培養液中に芳香性揮発物質の報告はなく、特にVM-A生成菌とその変異株による芳香性揮発物質の生成、VM-A生成、気菌糸の形成の関係に興味が持たれた。我々はこれらの微生物の発酵液からその揮発物質を分離し、同定を試みた。本報告では、VM-A生成菌からその揮発物質を分離・同定すると共にその変異株の揮発性物質、VM-Aの生成、既知抗生物質に対する感受性および気菌糸の形成の関係について述べる。 kn-abstract=Streptomyces hygroscopicus subsp. limoneus JCM 4911 , a validamycin producer was found to produce a volatile in the validamycin producing medium . The volatile was produced only by the organism which could form abundant aerial mycelium during the growth phase for one to two days of the culture and, thereafter, it decreased. Maximum production (3.88μg/ml) of the volatile was found in two-days' cultured broth with the conditions for validamycin production. On the contrary, the validamycin production started at around two days into the culture and increased up to five to seven days of the culture with maximum production of 440μg/ml. The volatile was extracted with n-pentane from the culture broth of S. hygroscopicus subsp. limoneus JCM 4911 and was identical with n-hexanol by GC-MS analysis. en-copyright= kn-copyright= en-aut-name=HigashideEiji en-aut-sei=Higashide en-aut-mei=Eiji kn-aut-name=東出栄治 kn-aut-sei=東出 kn-aut-mei=栄治 aut-affil-num=1 ORCID= en-aut-name=OhashiToshinari en-aut-sei=Ohashi en-aut-mei=Toshinari kn-aut-name=大橋利成 kn-aut-sei=大橋 kn-aut-mei=利成 aut-affil-num=2 ORCID= en-aut-name=HoqueMohamada Mahfuzul en-aut-sei=Hoque en-aut-mei=Mohamada Mahfuzul kn-aut-name=ホックモハマド マフズル kn-aut-sei=ホック kn-aut-mei=モハマド マフズル aut-affil-num=3 ORCID= en-aut-name=Najimashuhei en-aut-sei=Najima en-aut-mei=shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Streptomyces kn-keyword=Streptomyces en-keyword=volatile kn-keyword=volatile en-keyword=n-hexanol kn-keyword=n-hexanol en-keyword=validamycin kn-keyword=validamycin END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=13 end-page=17 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation and Purification of Nocardamine from the Validamycin Producer kn-title=バリダマイシン生成菌培養液から抗細菌性抗生物質ノカルダミンの分離・精製 en-subtitle= kn-subtitle= en-abstract=Streptomyces hygroscopicus subsp. limoneus はバリダマイシン生成菌として知られているが、その抗カビ物質以外に抗細菌性抗生物質を生成することは知られていない。われわれはその微生物がバリダマイシン非生成条件において生成する抗細菌性抗生物質を見出そうと試みた。その結果、グラム陰性細菌に活性を示す抗生物質EO-3を得たのでこれを分離・精製してノカルダミンと同定した。本報告では本抗生物質の培養条件、抗菌スペクトル、精製および同定方法について述べる。尚、この研究は平成6年度から8年度までの3年間に亙る岡山大学学内特定研究『特殊環境生物の機能開発と物質生産への応用』を分担して行ったものである。 kn-abstract=An antibacterial active against P. mirabilis was isolated from the culture of Streptomyces hygroscopicus subsp. limoneus, validamycin producer. The antibiotic was found to be produced with a non-validamycin producing condition. The antibiotic was identified as nocardamine with the analytical data, IR, 1H-NMR and 13C-NMR spectra. en-copyright= kn-copyright= en-aut-name=HigashideEiji en-aut-sei=Higashide en-aut-mei=Eiji kn-aut-name=東出栄治 kn-aut-sei=東出 kn-aut-mei=栄治 aut-affil-num=1 ORCID= en-aut-name=OmatsuYoshiharu en-aut-sei=Omatsu en-aut-mei=Yoshiharu kn-aut-name=大松義治 kn-aut-sei=大松 kn-aut-mei=義治 aut-affil-num=2 ORCID= en-aut-name=InoueSuemi en-aut-sei=Inoue en-aut-mei=Suemi kn-aut-name=井上末瑞 kn-aut-sei=井上 kn-aut-mei=末瑞 aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Streptomyces kn-keyword=Streptomyces en-keyword=antibacterial compoud kn-keyword=antibacterial compoud en-keyword=nocardamine kn-keyword=nocardamine en-keyword=non-validamycin producing condition kn-keyword=non-validamycin producing condition END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=1 article-no= start-page=5 end-page=8 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sn-1位に多価不飽和脂肪酸を結合する2-0-methoxyethoxymethylglycerolの立体配置決定 kn-title=Stereochemical Determination of 2-0-Methoxyethoxymethylglycerol Bearing Polyunsaturated Fatty Acyl Group at sn-1 Position en-subtitle= kn-subtitle= en-abstract=自然界に広く存在し，重要な生理機能を担っている多価不飽和脂肪酸結合リン脂質の化学的合成に必要な出発原料としての2-0-methoxyethoxymethylglycerolの立体配置と光学純度を，立体配置・光学純度既知の物質に化学的に誘導し，それらの比旋光度をお互いに比較する事により決定した． kn-abstract=A correlation of optical rotation, optical purity and configuration of the asymmetric center was done for 2-0-methoxyethoxymethylglycerol bearing polyunsaturated fatty acyl group at sn-1 position. This compound was enzymatically prepared and is an important starting material for the syntheses of optical active glycerophospholipids naving polyunwaturated fatty acyl groups. en-copyright= kn-copyright= en-aut-name=MatsumotoKotaro en-aut-sei=Matsumoto en-aut-mei=Kotaro kn-aut-name=松本好太郎 kn-aut-sei=松本 kn-aut-mei=好太郎 aut-affil-num=1 ORCID= en-aut-name=MoriYoshihiro en-aut-sei=Mori en-aut-mei=Yoshihiro kn-aut-name=森義裕 kn-aut-sei=森 kn-aut-mei=義裕 aut-affil-num=2 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=3 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=4 ORCID= en-aut-name=ShimizuSakayu en-aut-sei=Shimizu en-aut-mei=Sakayu kn-aut-name=清水昌 kn-aut-sei=清水 kn-aut-mei=昌 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=Division of Applied Life Science, Graduate School of Agriculture, Kyoto University en-keyword=phospholipid kn-keyword=phospholipid en-keyword=polyunsaturated fatty acid kn-keyword=polyunsaturated fatty acid en-keyword=methoxyethoxythoxymethylglycerol kn-keyword=methoxyethoxythoxymethylglycerol en-keyword=synthesis kn-keyword=synthesis END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=1 article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=多価不飽和脂肪酸を２位に結合するホスファチジルコリンの炭素アナローグの合成 kn-title=Synthesis of Carbon Analogues of Phosphatidylcholines Having a Polyunsaturated Fatty Acid at the 2-Position en-subtitle= kn-subtitle= en-abstract=自然界に広く存在するホスフォリパーゼA2はグリセロリン脂質の2位のエステル結合を選択的に切断する酵素であり,消化,アラキドン酸カスケードの起動,リン脂質過酸化物の代謝等,生理作用に広く関わっている。本研究ではホスフォリパーゼA2の基質ミメテイックとしてホスファチジルコリンの2位エステル結合が炭素-炭素結合に置き換わった化合物をアラキドン酸とステアリン酸を出発原料としてリパーゼ触媒によるアシル化反応及び有機化学反応によって合成した。 kn-abstract=Carbon analogues of Phosphatidylcholines having linoleic or arachidonic acid at the 2-position were synthesized. The synthetic route involves conversion of the polyunsaturated fatty acid iodination. The derivatives were converted to diols by LiAIH4 reduction and submitted to lipase-catalyzed monostearoylation in isopropylether. The mono-ester was converted to phoshatidylcholines by the usual phosphodiester synthesis. en-copyright= kn-copyright= en-aut-name=SekiChiyo en-aut-sei=Seki en-aut-mei=Chiyo kn-aut-name=勢喜千代 kn-aut-sei=勢喜 kn-aut-mei=千代 aut-affil-num=1 ORCID= en-aut-name=MoriYoshihiro en-aut-sei=Mori en-aut-mei=Yoshihiro kn-aut-name=森義裕 kn-aut-sei=森 kn-aut-mei=義裕 aut-affil-num=2 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=3 ORCID= en-aut-name=ShimizuSakayu en-aut-sei=Shimizu en-aut-mei=Sakayu kn-aut-name=清水昌 kn-aut-sei=清水 kn-aut-mei=昌 aut-affil-num=4 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=Division of Applied Bioscience, Graduate School of Agriculture, Kyoto University affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=phospholipid kn-keyword=phospholipid en-keyword=arachidonic acid kn-keyword=arachidonic acid en-keyword=synthesis kn-keyword=synthesis en-keyword=phospholipase A2 kn-keyword=phospholipase A2 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=1 article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=オリーブに含まれるオリーブアナアキゾウムシ摂食刺激物質β-sitostery-D-glucoside kn-title=β-SitosteryI-D-glucoside from the Olive Tree(Olea europaea LINNE; Oleaceae) as a Feeding Stimulant toward the Olive Weevil (Dyscerus perforatus) en-subtitle= kn-subtitle= en-abstract=オリーブアナアキゾウムシは，モクセイ科のオリーブに多数寄生し甚大な被害を与えるため，オリーブ栽培上の深刻な問題となっている．我々は，これまでオリーブのメタノール抽出物から，オリーブアナアキゾウムシの摂食刺激成分として，雌雄に活性を示すセコイリドイド配糖体1種と，雌に特異的に活性を持つ2種のリグナン類を得た．さらに今回，同じメタノール抽出物から，活性物質としてステロイド配糖体であるβ-sitosteryl-D-glucosideを得た．この成分は雌雄に対してほぼ同等の摂食刺激活性を示した． kn-abstract=The olive weevil [Dyscerus perforatus (ROELOFS); Coleopetera; Curculionidae] is a native species in Japan and now the most serious pest of the olive trees. Originally, this weevil seemed to colonise Ligustrum japonicum Thumb. and L. obtusifolium Sieb. et Zucc, both of which belong to the same oleacea family as olive. However, when olive trees were introduced to Japan in 1908, the weevils immediately attacked the plants and soon preferred them to the former hosts. Unlike in the former hosts, where the weevils live in a low population density, it is extraordinary high in the case of olive trees and the subsequent assault becomes seriously damaging for the host plant. During the course of our study on the relationship between olive trees and olive weevils, we came to be interested in the possible chemical constituents that are responsible for host selection and attraction of the olive weevil to this plant. Previously, we reported that a secoiridoid gluconside, oleuropein, and some lignans, (-)-olivil and (+)-l-acetoxypinoresinol, from the olive tree stimulated the feeding habit of the weevil. In this study, we found a steroidal glucoside as another feeding stimulant component in the olive tree. Here, we describe the isolation, characterization and activity of this feeding stimulant. en-copyright= kn-copyright= en-aut-name=KadowakiEmiko en-aut-sei=Kadowaki en-aut-mei=Emiko kn-aut-name=門脇英美子 kn-aut-sei=門脇 kn-aut-mei=英美子 aut-affil-num=1 ORCID= en-aut-name=YoshidaYasuhiro en-aut-sei=Yoshida en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=3 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=Nippon Olive Co., Ltd. affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Computer simulation kn-keyword=Computer simulation en-keyword=Selection kn-keyword=Selection en-keyword=Crossbreeding kn-keyword=Crossbreeding en-keyword=Population structure kn-keyword=Population structure END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=1 article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=200402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ageratum conyzoidesに含まれる殺線虫活性物質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Activity-guided chromatographic purification of the methanol extract of Ageratum conyzoides, using pine wood nematodes Bursaphelenchus xylophilus successfully led to the isolation and characterization of the nematicidal compound with minimum effective dose (MED) of 75 μg/ cotton ball (μg/bl.). Based on the chemical and spectral properties, the compound was determined to be coumarin (1). The activity of coumarin derivatives (2-5) was also investigated. en-copyright= kn-copyright= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=1 ORCID= en-aut-name=TakaishiKazuto en-aut-sei=Takaishi en-aut-mei=Kazuto kn-aut-name=高石和人 kn-aut-sei=高石 kn-aut-mei=和人 aut-affil-num=2 ORCID= en-aut-name=NaganoYouichi en-aut-sei=Nagano en-aut-mei=Youichi kn-aut-name=長野庸一 kn-aut-sei=長野 kn-aut-mei=庸一 aut-affil-num=3 ORCID= en-aut-name=AlenYohannes en-aut-sei=Alen en-aut-mei=Yohannes kn-aut-name=アレンヨハネス kn-aut-sei=アレン kn-aut-mei=ヨハネス aut-affil-num=4 ORCID= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=5 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Nematicidal compound kn-keyword=Nematicidal compound en-keyword=Ageratum conyzoides kn-keyword=Ageratum conyzoides en-keyword=Coumarin kn-keyword=Coumarin en-keyword=Cotton kn-keyword=Cotton END start-ver=1.4 cd-journal=joma no-vol=79 cd-vols= no-issue=1 article-no= start-page=1 end-page=35 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=グラヤノトキシンD環の化学修飾 kn-title=Introduction of Several Groups to the D-ring of Grayanotoxin en-subtitle= kn-subtitle= en-abstract= kn-abstract=Grayanotoxin (GTX), one of the lipid-soluble Na+ channel openers, contains four rings (A, B, C and D) and the chemical groups essential for the pharmacological action are located on the A- and B-rings. To study the biological significance of functional groups on the D-ring, 51 new derivatives were prepared from α-dihydro GTX- 11 . These new compounds and the previously prepared GTXS ere directly applied to the intracellular phase of internally perfused squid giant axons. en-copyright= kn-copyright= en-aut-name=IwasaJunkichi en-aut-sei=Iwasa en-aut-mei=Junkichi kn-aut-name=岩佐順吉 kn-aut-sei=岩佐 kn-aut-mei=順吉 aut-affil-num=1 ORCID= en-aut-name=KawanishiTetsuroh en-aut-sei=Kawanishi en-aut-mei=Tetsuroh kn-aut-name=川西徹朗 kn-aut-sei=川西 kn-aut-mei=徹朗 aut-affil-num=2 ORCID= en-aut-name=HandaSatoshi en-aut-sei=Handa en-aut-mei=Satoshi kn-aut-name=飯田聡 kn-aut-sei=飯田 kn-aut-mei=聡 aut-affil-num=3 ORCID= en-aut-name=KamanoHideki en-aut-sei=Kamano en-aut-mei=Hideki kn-aut-name=鎌野秀樹 kn-aut-sei=鎌野 kn-aut-mei=秀樹 aut-affil-num=4 ORCID= en-aut-name=YamamotoShingo en-aut-sei=Yamamoto en-aut-mei=Shingo kn-aut-name=山本真吾 kn-aut-sei=山本 kn-aut-mei=真吾 aut-affil-num=5 ORCID= en-aut-name=OkamotoManabu en-aut-sei=Okamoto en-aut-mei=Manabu kn-aut-name=岡本学 kn-aut-sei=岡本 kn-aut-mei=学 aut-affil-num=6 ORCID= en-aut-name=TakedaNorimasa en-aut-sei=Takeda en-aut-mei=Norimasa kn-aut-name=竹田典正 kn-aut-sei=竹田 kn-aut-mei=典正 aut-affil-num=7 ORCID= en-aut-name=NakamuraMikihiko en-aut-sei=Nakamura en-aut-mei=Mikihiko kn-aut-name=中村幹彦 kn-aut-sei=中村 kn-aut-mei=幹彦 aut-affil-num=8 ORCID= en-aut-name=MasutaniTetsuya en-aut-sei=Masutani en-aut-mei=Tetsuya kn-aut-name=桝谷哲也 kn-aut-sei=桝谷 kn-aut-mei=哲也 aut-affil-num=9 ORCID= en-aut-name=ShiroMotoo en-aut-sei=Shiro en-aut-mei=Motoo kn-aut-name=城始勇 kn-aut-sei=城 kn-aut-mei=始勇 aut-affil-num=10 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=11 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=12 ORCID= en-aut-name=SeyamaIssei en-aut-sei=Seyama en-aut-mei=Issei kn-aut-name=瀬山一正 kn-aut-sei=瀬山 kn-aut-mei=一正 aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=Technical R & D Division, Terumo Co. affil-num=3 en-affil= kn-affil=Niigata Refinery, Showa Shell Sekiyu Co. affil-num=4 en-affil= kn-affil=Central Research Laboratories, Idemitsu Kosan Co. affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=Tondabayashi High School affil-num=8 en-affil= kn-affil=Kao Institute for Fundamental Research, Kao Co. affil-num=9 en-affil= kn-affil=Chemical Division, Daikin Industries Co. affil-num=10 en-affil= kn-affil=Shionogi Reserch Laboratries, Shionogi & Co. Ltd. affil-num=11 en-affil= kn-affil=岡山大学 affil-num=12 en-affil= kn-affil=岡山大学 affil-num=13 en-affil= kn-affil=School of Medicine, Hiroshima University END