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ID 34241
FullText URL
Author
Toyoshima, Megumi
Hosoda, Kanako
Hanamura, Miho
Kobayashi, Hiroshi
Abstract

Numerous epidemiological investigations show that sunlight is carcinogenic to humans and that the use of sunscreen may be effective in decreasing the risk of skin cancer. The biological activity of a sunscreen is evaluated by its ability to protect human skin from erythema as represented by a Sun Protection Factor (SPF). We propose that the sunscreen's protective effect against sunlight-induced genotoxicity, including mutation, should also be taken into account. In this study we examined the protective ability of sunscreens against natural sunlight and UV-induced genotoxicity in Drosophila somatic cells. We prepared three kinds of sunscreen samples, each with an SPF value of 20, 40 or 60 and compared their protective activities with commercial sunscreens. When a sunscreen of SPF 20, 40 or 60 was pasted on the plastic cover of a petri dish in which Drosophila larvae were exposed to the sun or UV lamps, genotoxicity decreased as the SPF of the sunscreen increased, relative to levels of genotoxicity observed in samples without sunscreen. However, the protective abilities of sunscreens were unexpectedly not so different from each other. To reveal the relationship between the protective activity of sunscreen and the wavelength of light with which larvae were irradiated through the sunscreen, we measured the transmittance of light through the petri dish cover on which the sunscreen was pasted. Effective protection was demonstrated by removing components of light whose wavelengths were below 315 nm. We suggest, that the measurement of anti-genotoxic activity and the determination of the wavelengths of light transmitted through the sunscreen should be an alternative method for evaluating the effectiveness of a sunscreen.

Keywords
sunscreen
sunlight
UVB
somatic cell mutation
genotoxicity
transmittance
Drosophila melanogaster
Note
Digital Object Identifer:10.1016/j.jphotobiol.2003.09.005
Published with permission from the copyright holder. This is the institute's copy, as published in Journal of Photochemistry and Photobiology B-Biology, November 2003, Volume 73, Issue 1-2, Pages 59-66.
Publisher URL:http://dx.doi.org/10.1016/j.jphotobiol.2003.09.005
Direct access to Thomson Web of Science record
Copyright © 2003 Elsevier B.V. All rights reserved.
Published Date
2003-11
Publication Title
Journal of Photochemistry and Photobiology B: Biology
Volume
volume73
Issue
issue1-2
Start Page
59
End Page
66
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Science KeyUT
Submission Path
biochemistry/3