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ID 48980
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Author
Kuroda, Yuka
Yamashita, Keisuke
Abstract
Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.
Keywords
Adsorption
Porcine sperm motility
Silicone elastomer
Trajectories
Published Date
2010-10-12
Publication Title
Journal of Reproduction and Development
Volume
volume57
Issue
issue1
Publisher
Society for Reproduction and Development
Start Page
163
End Page
167
ISSN
0916-8818
Content Type
Journal Article
Project
Research Core for Interdisciplinary Sciences
Official Url
https://www.jstage.jst.go.jp/article/jrd/57/1/57_10-075N/_article
language
英語
Copyright Holders
Copyright © 2011 Society for Reproduction and Development
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Refereed
True
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