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ID 48975
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Author
Koike, Takayuki
Naruse, Keiji Kaken ID researchmap
Abstract
Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P<0.05). These observations indicate that tilting culture during IVM and IVC in chemically defined media improves the quality of blastocyst, as determined by the number of cells per blastocyst, without any effects on penetrability and developmental competence.
Keywords
Inclining device
In vitro culture
In vitro fertilization
Oocytes
Pig
Published Date
2010-10
Publication Title
Journal of Reproduction and Development
Volume
volume56
Issue
issue5
Publisher
Society for Reproduction and Development
Start Page
552
End Page
557
ISSN
0916-8818
Content Type
Journal Article
Project
Research Core for Interdisciplinary Sciences
Official Url
https://www.jstage.jst.go.jp/article/jrd/56/5/56_10-041H/_article
language
英語
Copyright Holders
Copyright © 2010 Society for Reproduction and Development
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Refereed
True
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