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ID 57788
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Toba, Shinsuke Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Minato, Yusuke Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID publons
Kondo, Yuma Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Hoshikawa, Kanami Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Minagawa, Shu
Komaki, Shiho Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kumagai, Takanori Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Matoba, Yasuyuki Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Morita, Daichi Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Ogawa, Wakano Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Gotoh, Naomasa Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University
Tsuchiya, Tomofusa Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kuroda, Teruo Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Abstract
We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized systems, sdeXY, sdeAB, and sdeCDE. Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain of Escherichia coil. Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance in S. marcescens. The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6G efflux assay. When expressed in the toiC-deficient strain of E. coil, KAM43, none of the genes conferred resistance on E. coil. When hasF, encoding the S. marcescens ToIC ortholog, was expressed in KAM43, all of the genes conferred resistance on E. coil, suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed a sdeXY deletion mutant from a derivative strain of the clinically isolated multidrug-resistant S. marcescens strain and found that the sdeXY deletion mutant was sensitive to a broad spectrum of antimicrobial agents.
Published Date
2019-3-19
Publication Title
Scientific Reports
Volume
volume9
Publisher
Nature Publishing Group
Start Page
4854
ISSN
2045-2322
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2019
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DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1038/s41598-019-41237-7
License
https://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
JP24590080