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ID 32981
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Author
Asano, Masahiro
Nakanishi, Tohru
Yamaai, Tomoichiro
Yosimichi, Gen
Ohyama, Kazumi
Murayama, Yoji
Takigawa, Masaharu Kaken ID publons researchmap
Abstract

Background: CCN2/CTGF is known to be involved in tooth germ development and periodontal tissue remodeling, as well as in mesenchymal tissue development and regeneration. In this present study, we investigated the roles of CCN2/CTGF in the proliferation and differentiation of periodontal ligament cells (murine periodontal ligament-derived cell line: MPL) in vitro.
Results: In cell cultures of MPL, the mRNA expression of the CCN2/CTGF gene was stronger in sparse cultures than in confluent ones and was significantly enhanced by TGF-β. The addition of Recombinant CCN2/CTGF (rCCN2) to MPL cultures stimulated DNA synthesis and cell growth in a dose-dependent manner. Moreover, rCCN2 addition also enhanced the mRNA expression of alkaline phosphatase (ALPase), type I collagen, and periostin, the latter of which is considered to be a specific marker of the periosteum and periodontium; whereas it showed little effect on the mRNA expression of typical osteoblastic markers, e.g., osteopontin and osteocalcin. Finally, rCCN2/CTGF also stimulated ALPase activity and collagen synthesis.
Conclusion: These results taken together suggest important roles of CCN2/CTGF in the development and regeneration of periodontal tissue including the periodontal ligament.

Note
Digital Object Identifier:10.1186/1478-811X-3-11
Published with permission from the copyright holder. This is the institute's copy, as published in Cell Communication and Signaling, Volume 3, 5 October 2005, Article number 11.
Publisher URL:http://dx.doi.org/10.1186/1478-811X-3-11
Copyright © 2005 Asano et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Published Date
2005-10-05
Publication Title
Cell Communication and Signaling
Volume
volume3
Issue
issue1
Publisher
BioMed Central
NCID
AA12050544
Content Type
Journal Article
language
英語
Copyright Holders
Asano et al; licensee BioMed Central Ltd.
File Version
publisher
Refereed
True
DOI
PubMed ID
Submission Path
biology_general/2