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Jinno, Katsuya Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hiramatsu, Bunta Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Tsunashima, Kenta Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Fujimoto, Kayo Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Sakai, Kenji Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID
Kiwa, Toshihiko Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Tsukada, Keiji Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID researchmap
Abstract
Liquid-phase magnetic immunoassay (MIA) using magnetic nano-particles (MNPs) has been studied as a more rapid method compared to optical methods for inspecting proteins and viruses. MIA can estimate the number of conjugated antibodies without being washed differently from conventional optical immunoassay. However, in the case of the liquid phase, it is considered that the magnetic properties of MNPs are affected by physical properties such as viscosity and impurity substances such as biological substances contained in the blood. In this study, the effect of sodium chloride (NaCl) in buffer and serum solution was evaluated to reveal the effect of serum because the sodium (Na+) and chloride (Cl-) ions in the serum dominate ion balance of blood. The measurement results of AC magnetic susceptibility and a dynamic light scattering (DLS) showed that the aggregation of MNPs was largely affected by the concentration of NaCl. This effect of the NaCl could be explained by shielding of the surface charge of MNPs by ions in the solution. Although the concentrations of NaCl in the buffer and serum solution were almost same, we found that MNPs were aggregated more in their size for those in the serum solution because of other impurities, such as proteins. These results suggest evaluation of effects of the contaminants in serum and optimization of polymer coatings of MNPs could be important factors to realize measurements of magnetic immunoassay with high accuracy. (C) 2019 Author(s).
Published Date
2019-12-20
Publication Title
AIP Advances
Volume
volume9
Issue
issue12
Publisher
American Institute of Physics
Start Page
125317
ISSN
2158-3226
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© Author(s) 2019
File Version
publisher
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1063/1.5130168
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
JP15H05764