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Author
Afify, Said M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University ORCID
Calle, Anna Sanchez Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
Hassan, Ghmkin Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID publons
Kumon, Kazuki Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Nawara, Hend M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Zahra, Maram H. Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Mansour, Hager M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Khayrani, Apriliana Cahya Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Alam, Md Jahangir Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Du, Juan Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Seno, Akimasa Okayama University Research Laboratory of Stem Cell Engineering in Detroit, IBio, Wayne State University ORCID Kaken ID publons researchmap
Iwasaki, Yoshiaki Department of Gastroenterology and Hepatology, Graduate School of Medicine, Okayama University ORCID Kaken ID publons researchmap
Seno, Masaharu Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
Abstract
Background
Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown.
Methods
In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed.
Results
The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers.
Conclusions
We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.
Keywords
Cancer models
Cancer stem cells
Published Date
2020-03-17
Publication Title
British Journal of Cancer
Volume
volume122
Issue
issue9
Publisher
Springer Nature
Start Page
1378
End Page
1390
ISSN
0007-0920
NCID
AA00574355
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© Authors
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1038/s41416-020-0792-z
License
http://creativecommons. org/licenses/by/4.0/