このエントリーをはてなブックマークに追加
ID 61045
Author
Osman, Amira Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems
Oze, Miharu Department of Medical Bioengineering, Graduate School of Natural Science and Technology
Afify, Said M. Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems ORCID
Hassan, Ghmkin Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems ORCID publons
EL-Ghlban, Samah Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Nawara, Hend M. Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Fu, Xiaoying Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Zahra, Maram Hussein Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Seno, Akimasa Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Winer, Ira Department of Obstetrics & Gynecology, School of Medicine, Wayne State University
Salomon, David S. Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute
Seno, Masaharu Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Abstract
Macrophages are the most abundant immune cells in the microenvironment of solid tumors. The present study displayed histological and immunohistochemical analyses of a malignant tumor model developed from cancer stem cells (CSCs) converted from human induced pluripotent stem cells (hiPSCs) in a cancer microenvironment prepared from the conditioned medium (CM) of a pancreatic cancer cell line. We focused on the localization and the origin of tumor-associated macrophages (TAMs), To the best of our knowledge this may be the first study to suggest the potential differentiation of CSCs to TAMs.hiPSCs were converted into CSCs in the presence of CM from PK8 cells. CSCs were then transplanted in vivo and formed primary tumors. Primary cultures for these tumors were serially transplanted again to obtain secondary tumors. Secondary tumors exhibited histopathological features of malignancy. Cells derived from tumors maintained the expression of endogenous stemness markers and pancreatic CSCs markers. Simultaneously, high immunoreactivity to anti-mouse CD68, anti-human CD68, CD206 and CD11b antibodies were detected revealing that the tumor tissue derived from CSCs was enriched for macrophages which can originate from both human and mouse cells.The model of CSCs highlighted the possibility of CSCs to differentiate into TAMs.
Keywords
TAMs
TME
hiPSCs
Anti-CD antibodies
Note
This fulltext is available in September, 2021.
Published Date
2020-12-31
Publication Title
Acta Histochemica
Volume
volume122
Issue
issue8
Publisher
Elsevier
Start Page
151628
ISSN
0065-1281
NCID
AA00508000
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1016/j.acthis.2020.151628
License
https://creativecommons.org/licenses/by-nc-nd/4.0/