FullText URL IJO54_1_283.pdf
Author Shimizu, Rieko| Ibaragi, Soichiro| Eguchi, Takanori| Kuwajima, Daisuke| Kodama, Shinichi| Nishioka, Takashi| Okui, Tatsuo| Obata, Kyoichi| Takabatake, Kiyofumi| Kawai, Hotaka| Ono, Kisho| Okamoto, Kuniaki| Nagatsuka, Hitoshi| Sasaki, Akira|
Keywords nicotine head and neck squamous cell carcinoma lymph node metastasis cetuximab
Published Date 2018-11-12
Publication Title International Journal of Oncology
Volume volume54
Issue issue1
Publisher Spandidos Publications
Start Page 283
End Page 294
ISSN 1019-6439
NCID AA10992511
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version publisher
PubMed ID 30431077
DOI 10.3892/ijo.2018.4631
Web of Science KeyUT 000451845400025
Related Url isVersionOf https://doi.org/10.3892/ijo.2018.4631
JaLCDOI 10.18926/AMO/50408
FullText URL 67_3_153.pdf
Author Yamamoto, Masanao| Fujita, Hirofumi| Katase, Naoki| Inoue, Keiji| Nagatsuka, Hitoshi| Utsumi, Kozo| Sasaki, Junzo| Ohuchi, Hideyo|
Abstract Ever since protoporphyrin IX (PpIX) was discovered to accumulate preferentially in cancer cells after 5-aminolevulinic acid (ALA) treatment, photodynamic treatment or therapy (PDT) has been developed as an exciting new treatment option for cancer patients. However, the level of PpIX accumulation in oral cancer is fairly low and insufficient for PDT. Ferrochelatase (FECH) and ATP-binding cassette transporter G2 (ABCG2) are known to regulate PpIX accumulation. In addition, serum enhances PpIX export by ABCG2. We investigated here whether and how inhibitors of FECH and ABCG2 and their combination could improve PpIX accumulation and PDT efficacy in an oral cancer cell line in serum-containing medium. ABCG2 inhibitor and the combination of ABCG2 and FECH inhibitors increased PpIX in the presence of fetal bovine serum (FBS) in an oral cancer cell line. Analysis of ABCG2 gene silencing also revealed the involvement of ABCG2 in the regulation of PpIX accumulation. Inhibitors of FECH and ABCG2, and their combination increased the efficiency of ALA-PDT even in the presence of FBS. ALA-PDT-induced cell death was accompanied by apoptotic events and lipid peroxidation. These results suggest that accumulation of PpIX is determined by the activities of ABCG2 and FECH and that treatment with a combination of their inhibitors improves the efficacy of PDT for oral cancer, especially in the presence of serum.
Keywords 5-aminolevulinic acid protoporphyrin IX oncology photodynamic therapy apoptosis
Amo Type Original Article
Published Date 2013-06
Publication Title Acta Medica Okayama
Volume volume67
Issue issue3
Publisher Okayama University Medical School
Start Page 153
End Page 164
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 23804138
Web of Science KeyUT 000320747900004
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/50681
Author Yanagi, Yoshinobu| Fujita, Mariko| Hisatomi, Miki| Matsuzaki, Hidenobu| Konouchi, Hironobu| Katase, Naoki| Nagatsuka, Hitoshi| Asaumi, Jun-ichi|
Published Date 201012
Publication Title Oral Radiology
Volume volume26
Issue issue2
Content Type Journal Article
Author Maki, Yu| Murakami, Jun| Asaumi, Jun-ichi| Tsujigiwa, Hidetsugu| Nagatsuka, Hitoshi| Kokeguchi, Susumu| Fukui, Kazuhiro| Kawai, Noriko| Yanagi, Yoshinobu| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
Published Date 2005-7
Publication Title Oral Oncology
Volume volume41
Issue issue10
Content Type Journal Article
Author Murakami, Jun| Asaumi, Jun-ichi| Kawai, Noriko| Tsujigiwa, Hidetsugu| Yanagi, Yoshinobu| Nagatsuka, Hitoshi| Inoue, Tetsuyoshi| Kokeguchi, Susumu| Kawasaki, Shoji| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
Published Date 2005-07
Publication Title Cancer Chemotherapy and Pharmacology
Volume volume56
Issue issue1
Content Type Journal Article
Author Watanabe, Nobuyuki| Takaoka, Munenori| Sakurama, Kazufumi| Tomono, Yasuko| Hatakeyama, Shinji| Ohmori, Osamu| Motoki, Takayuki| Shirakawa, Yasuhiro| Yamatsuji, Tomoki| Haisa, Minoru| Matsuoka, Junji| Beer, David G.| Nagatsuka, Hitoshi| Tanaka, Noriaki| Naomoto, Yoshio|
Published Date 2010-04-01
Publication Title 岡山医学会雑誌
Volume volume122
Issue issue1
Content Type Journal Article
Author 長塚 仁|
Published Date 1991-03-28
Publication Title
Content Type Thesis or Dissertation