Author Satoh, Daisuke| Yagi, Takahito| Nagasaka, Takeshi| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Utsumi, Masashi| Tanaka, Takehiro| Sadamori, Hiroshi| Fujiwara, Toshiyoshi|
Published Date 2013-04-27
Publication Title World Journal of Hepatology
Volume volume5
Issue issue4
Content Type Journal Article
Author Nobuoka, Daisuke| Yoshikawa, Toshiaki| Takahashi, Mari| Iwama, Tatsuaki| Horie, Kazutaka| Shimomura, Manami| Suzuki, Shiro| Sakemura, Noriko| Nakatsugawa, Munehide| Sadamori, Hiroshi| Yagi, Takahito| Fujiwara, Toshiyoshi| Nakatsura, Tetsuya|
Published Date 2013-04
Publication Title Cancer Immunology, Immunotherapy
Volume volume62
Issue issue4
Content Type Journal Article
JaLCDOI 10.18926/AMO/49671
FullText URL 67_2_117.pdf
Author Sadamori, Hiroshi| Yagi, Takahito| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Sato, Daisuke| Nobuoka, Daisuke| Utsumi, Masashi| Fujiwara, Toshiyoshi|
Abstract We present a case of living donor liver transplantation to a 3-year disease-free survivor of liver resection for hepatocellular carcinoma (HCC) with major portal vein invasion. A 48-year-old man had HCC in the right lobe with a portal venous tumor thrombus extending into the left portal vein. An extended right lobectomy with thrombectomy was performed to remove the thrombus. Three years after liver resection, the patient experienced liver failure, with massive ascites and jaundice due to the formation of a thrombus in the main and left portal veins. During the 3 years after liver resection, no metastasis or recurrence of HCC had been detected, and tumor markers had been within normal ranges. The portal venous thrombus did not show any arterial enhancement under contrast-enhanced computed tomography, suggesting that the co-existence of any HCC component in the portal venous thrombus may have been negative. Based on these findings, living donor liver transplantation was performed using a right lobe graft from the patientʼs son. The patient is alive at 87 months after the transplantation, with no evidence of HCC recurrence.
Keywords living donor liver transplantation hepatocellular carcinoma portal vein invasion liver resection
Amo Type Case Report
Published Date 2013-04
Publication Title Acta Medica Okayama
Volume volume67
Issue issue2
Publisher Okayama University Medical School
Start Page 117
End Page 121
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 23603929
Web of Sience KeyUT 000317801700007
JaLCDOI 10.18926/AMO/48268
FullText URL 66_2_177.pdf.pdf
Author Utsumi, Masashi| Matsuda, Hiroaki| Sadamori, Hiroshi| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Satoh, Daisuke| Hashimoto, Masaaki| Yagi, Takahito| Fujiwara, Toshiyoshi|
Abstract We report 4 cases of surgical resection of metachronous lymph node (LN) metastases from hepatocellular carcinoma (HCC) following hepatectomy. Clinicopathological features and results of LN dissection were investigated in the 4 patients. One patient was found to have a single metastasis in the mediastinal LNs, another had multiple metastases in the mediastinal and abdominal LNs, and the other 2 had single metastases in the abdominal LN. The locations of the abdominal LN metastases were behind the pancreas head in 2 patients and around the abdominal aorta in 1 patient. They all underwent surgical resection of metastatic LNs and had no postoperative complications. The 3 patients whose LN metastases were solitary have been alive for more than 2 years after LN resection, and one of them is free from recurrence. The patient with multiple LN metastases died 13 months after LN resection due to carcinomatosis. With the expectation of long-term survival, a single metachronous LN metastasis from HCC after hepatectomy should be resected in patients without uncontrollable intrahepatic or extrahepatic tumors.
Keywords hepatocellular carcinoma lymph node metastasis hepatectomy
Amo Type Case Report
Published Date 2012-04
Publication Title Acta Medica Okayama
Volume volume66
Issue issue2
Publisher Okayama University Medical School
Start Page 177
End Page 182
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22525476
Web of Sience KeyUT 000303175300011
Author Shinoura, Susumu| Yagi, Takahito| Sadamori, Hiroshi| Matsuda, Hiroaki| Umeda, Yuzo| Yoshida, Ryuichi| Satoh, Daisuke| Utsumi, Masashi| Yokomichi, Naosuke| Kuise, Takashi| Fujiwara, Toshiyoshi|
Published Date 2012-04-01
Publication Title 岡山医学会雑誌
Volume volume124
Issue issue1
Content Type Journal Article
Author Satoh, Daisuke| Yagi, Takahito| Sadamori, Hiroshi| Umeda, Yuzo| Fujiwara, Toshiyoshi|
Published Date 2011-12-01
Publication Title 岡山医学会雑誌
Volume volume123
Issue issue3
Content Type Journal Article
Author Satoh, Daisuke| Yagi, Takahito| Sadamori, Hiroshi| Matsuda, Hiroaki| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Utsumi, Takashi| Fujiwara, Toshiyoshi|
Published Date 2011-12-01
Publication Title 岡山医学会雑誌
Volume volume123
Issue issue3
Content Type Journal Article
JaLCDOI 10.18926/AMO/32828
FullText URL fulltext.pdf
Author Liu, Jie| Yagi, Takahito| Sadamori, Hiroshi| Matsukawa, Hiroyoshi| Sun, Dong-Sheng| Mitsuoka, Naoshi| Yamamura, Masao| Matsuoka, Junji| Jin, Zaishun| Yamamoto, Itaru| Tanaka, Noriaki|
Abstract <p>Controversy exists over whether the predominant cell death of hepatocytes is due to apoptosis or necrosis after ischemia/reperfusion injury. In this study we investigated the predominant cell death of hepatocytes after cold ischemia/reperfusion injury using the Annexin V-based assay, and evaluated the anti-apoptotic effect of ascorbic acid 2-glucoside (AA-2G) added to the University of Wisconsin solution (UW solution) in rat liver transplantation. The retrieved liver was preserved in 4 UW solution for 24 h, and then transplanted orthotopically to the syngeneic Wistar recipient. The animals were divided into 2 groups, a control group (n=10), in which liver grafts were preserved in UW solution (4), and an AA-2G group (n=10), in which liver grafts were preserved in UW solution (4) with AA-2G (100 ug/ml). The serum AST level 4 h after reperfusion in the control group was significantly suppressed in the AA-2G group, and the bile production of the liver graft in the AA-2G group was well recovered. The mean survival time in the AA-2G group was significantly improved compared with that in the control group. Annexin-V and Propidium iodide staining 4 h after reperfusion showed a significantly higher percentage of viable hepatocytes in the AA-2G group compared with the control group (93.4 +/- 2.0 vs. 80.3 +- 2.1%, P&#60;0.05). In the control group, the main cell death of hepatocytes was apoptosis (early apoptosis: 10.0 +- 4.7%, late apoptosis: 6.4 +/- 1.7%). The addition of AA-2G to the UW solution significantly inhibited both early and late apoptotic cell death 4 h after reperfusion (early apoptosis: 0.98 +/- 0.88%, late apoptosis: 2.2 +/- 1.1%). The expression of caspase 9 in the immunostaining of the liver graft was suppressed in the AA-2G group compared with in the control group. Our study using the Annexin V-based assay provided evidence that the predominant cell death of hepatocytes was apoptosis after 24 h cold ischemia/reperfusion injury in rat liver transplantation. The addition of AA-2G to the UW solution attenuated 24 h cold ischemia/reperfusion injury by inhibiting the apoptosis of hepatocytes.</p>
Keywords apoptosis ischemia/ reperfusion injury liver transplantation ascorbic acid 2- glucoside(AA-2G)
Amo Type Article
Published Date 2003-10
Publication Title Acta Medica Okayama
Volume volume57
Issue issue5
Publisher Okayama University Medical School
Start Page 209
End Page 216
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 14679398
Web of Sience KeyUT 000186186000001
JaLCDOI 10.18926/AMO/32294
FullText URL fulltext.pdf
Author Kuinose, Masahiko| Iwagaki, Hiromi| Morimoto, Yoshinori| Kohka, Hideo| Kobashi, Kenta| Sadamori, Hiroshi| Inagaki, Masaru| Urushihara, Naoto| Yagi, Takahito| Tanaka, Noriaki|
Abstract <p>Tacrolimus (FK-506) and cyclosporin A (CsA) are calcineurin antagonists used widely as T-cell immunosuppressants; however, their relative efficacy on the production of interleukin-18 (IL-18) remains undefined. We have examined the effects of FK-506 and CsA on the cytokine generation of human peripheral blood mononuclear cells (PBMCs) in mixed lymphocyte reaction (MLR) with lipopolysaccharide (LPS). We studied the levels of interleukin-18 (IL-18), IL-12, IL-10, IL-6, IL-2 and interferon-gamma (IFN-gamma) in the supernatant in allo-MLR by ELISA assay. Supernatant levels of IFN-gamma, IL-2, IL-6, IL-10 and IL-12 were detected 12 h after MLR and markedly increased thereafter. In contrast, production of IL-18 was detected at 12 h, reached a near maximum level at 24 h and decreased at 72 h. These results suggested that IFN-gamma production depended on IL-18, IL-12 and IL-2 in the early phase of MLR and depended mainly on IL-12 and IL-2 in the late phase. Both calcineurin antagonists inhibit the generation of IL-18, which plays a large role in allogeneic cell interactions, in macrophages and they also promote an equivalent down-regulation of T helper 1 (Th1) and Th2 responses in a concentration-dependent manner. About 90% of IFN-gamma production induced by MLR was inhibited by an anti-IL-18 antibody, showing that IL-18 can trigger IFN-gamma production in MLR. These results suggest that dual signaling consisting of antigen-driven nuclear factor of activated T cells (NFAT) activation and LPS-mediated NF-kappaB activation is crucial for IL-18 production in macrophages, and that IL-18 can trigger IFN-gamma production in T-cells by MLR.</p>
Keywords tacrolimus cyclosporin calcineurin antagonist
Amo Type Article
Published Date 2000-10
Publication Title Acta Medica Okayama
Volume volume54
Issue issue5
Publisher Okayama University Medical School
Start Page 201
End Page 209
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11061569
Web of Sience KeyUT 000090098600003
Author Yagi, Takahito| Urushihara, Naoto| Oishi, Masahiro| Matsukawa, Hiroyoshi| 藤原 俊哉| 松野 剛| Mori, Masanobu| Tsuge, Hiromu| Takakura, Norihisa| Tanaka, Noriaki| Higashi, Toshihiro| Tsuji, Takao| Inagaki, Masaru| Sadamori, Hiroshi| Kamikawa, Yasuaki|
Published Date 1998-06-25
Publication Title 岡山医学会雑誌
Volume volume110
Issue issue1-6
Content Type Journal Article
Author 貞森 裕|
Published Date 1996-03-25
Publication Title
Content Type Thesis or Dissertation