FullText URL fulltext.pdf
Author Ohtsuki, Takashi| Hatipoglu, Omer F.| Asano, Keiichi| Inagaki, Junko| Nishida, Keiichiro| Hirohata, Satoshi|
Keywords cell migration-inducing hyaluronidase 1 (CEMIP) chondrocyte hyaluronan mechanical strain nuclear factor kappa B (NF-kappa B) osteoarthritis
Published Date 2020-04-29
Publication Title International Journal of Molecular Sciences
Volume volume21
Issue issue9
Publisher MDPI
Start Page 3140
ISSN 1422-0067
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © 2020 by the authors.
File Version publisher
PubMed ID 32365591
DOI 10.3390/ijms21093140
Web of Science KeyUT 000535581700111
Related Url isVersionOf https://doi.org/10.3390/ijms21093140
JaLCDOI 10.18926/AMO/57957
FullText URL 74_1_77.pdf
Author Endo, Hirosuke| Akazawa, Hirofumi| Yashiro, Masato| Yamada, Kazuki| Sanki, Tomoaki| Tetsunaga, Tomonori| Nishida, Keiichiro| Furumatsu, Takayuki| Ozaki, Toshifumi|
Abstract Idiopathic chondrolysis of the hip (ICH), a very rare disorder of unknown etiology, occurs mainly in female adolescents. Characterized by pain, limp, stiffness and radiological narrowing joint space from the rapid destruction of the articular cartilage, ICH sometimes results in ankyloses. We present the case of a 10-year-old girl diagnosed with ICH based on arthroscopic inspection and synovium biopsy. The femoral deformity appeared gradually, like a cam-type femoroacetabular impingement. She was treated with intensive rehabilitation and immunosuppressive drug. We later performed an arthroscopic bumpectomy for residual symptoms. She achieved a favorable outcome as a 15-year-old at the latest follow-up.
Keywords idiopathic chondrolysis hip joint medication bump arthroscopy
Amo Type Case Report
Published Date 2020-02
Publication Title Acta Medica Okayama
Volume volume74
Issue issue1
Publisher Okayama University Medical School
Start Page 77
End Page 81
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2020 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 32099253
reference Waldenstrom H : On necrosis of the joint cartilage by epiphyseolysis capitis femoralis. Acta Chir Scand (1930) 67: 936-946.| Jones BS: Adolescent chondrolysis of the hip joint. South African Med J (1971) 45: 196-202.| Duncan JW, Nasca R and Schrantz J: The bizarre stiff hip. Possible Idiopathic Chondrolysis. J Am Med Assoc (1975) 231: 382-385.| Segaren N, Abdul-Jabar, Segaren N and Hashemi-Nejad A: Idiopathic chodrolysis of the hip: presentation, natural history and treatment options. J Pediatr Orthop B (2014) 23: 112-116.| Hoven H, Keessen W and Kuis W: Idiopathic chondrolysis of the hip; a distinct clinical entity ? Acta Orthop Scand (1989) 60: 661-663.| Dechosilpa C, Mulpruek P, Woratanarat P and Thiabratana P: Idiopathic chondrolysis of hip. Idiopathic Chondrolysis of Hip (ICH): report of three cases. Malaysian Orthopaedic J (2014) 8: 30-32.| Sureka J, Jakkani RK, Inbaraji A and Panwar S: Idiopathic chondrolysis of hip. Jpn J Radiol (2011) 29: 283-285.| Daluga DJ and Millar EA: Idipathic chondrolysis of the hip. J Pediatr Orthop (1989) 9: 405-411.| Johnson K, Haigh SF, Ehtisham S, Ryder C and Gardner-Medwin J: Childhood idiopathic chondrolysis of the hip; MRI features. Pediatr Radiol (2003) 33: 194-199.| Laor T and Crawford AH: Idiopathic chondrolysis of the hip in children; early MRI findings. AJR (2009) 192: 526-531.| Khoshhal KI, Awaad Y and Abbak AA: Botulinum neurotoxin-A in idiopathic chondrolysis; a case report of two cases. J Pediatr Orthop B (2014) 23: 441-446.| Picazo DR, Perez FD, Ortega PJ and Aparicio NG: An usual case of chondrolysis of the hip; a possible etiology for a rare condition - a case report. J Pediatr Orthop B (2016) 25: 533-538.| Appleyard DV, Schiller JR, Eberson CP and Ehrlich MG: Idiopathic chondrolysis treated with eternercept. Orthopedics (2009) 32: 214‒217.| Roy DR and Crawford AH: Idiopathic chondrolysis of the hip; management by subtotal capsulectomy and aggressive rehabilitation. J Pediatr Orthop (1988) 8: 203-207.| Garcia ADC, Fernandez PL, Gonzalez MPC, Garcia AC, Gonzalez LR and Jimenez JP: Idiopathic chondrolysis of the hip; long term evolution. J Pediatr Orthop (1999) 19: 449-454.|
JaLCDOI 10.18926/AMO/57720
FullText URL 73_6_537.pdf
Author Takahara, Yasuhiro| Nishida, Keiichiro| Nakashima, Hirotaka| Ochi, Nobuaki| Uchida, Yoichiro| Kato, Hisayoshi| Itani, Satoru| Nakamura, Makoto| Iwasaki, Yuichi| Tsujimura, Yoshitaka|
Abstract High tibial osteotomy (HTO) procedure is generally contraindicated in rheumatoid arthritis (RA) patients because synovial inflammation may exacerbate joint damage post-surgery. The natural course of joint destruction in RA changed dramatically with new treatment strategies and the introduction of biologic disease-modifying anti-rheumatic drugs (bDMARDs). We report the cases of two RA patients who underwent HTO and whose disease activities were well controlled by bDMARDs. Despite their short follow-up periods, they showed acceptable objective and subjective clinical results. We believe that the combination of bDMARDs and HTO can be indicated for selected RA patients before total knee arthroplasty.
Keywords high tibial osteotomy rheumatoid arthritis biologic DMARD knee surgery
Amo Type Case Report
Published Date 2019-12
Publication Title Acta Medica Okayama
Volume volume73
Issue issue6
Publisher Okayama University Medical School
Start Page 537
End Page 542
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2019 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 31871338
Web of Science KeyUT 000503431400011
FullText URL fulltext.pdf
Author Ohtsuki, Takashi| Shinaoka, Akira| Kumagishi-Shinaoka, Kanae| Asano, Keiichi| Hatipoglu, Omer Faruk| Inagaki, Junko| Takahashi, Ken| Oohashi, Toshitaka| Nishida, Keiichiro| Naruse, Keiji| Hirohata, Satoshi|
Published Date 2019-10-15
Publication Title Experimental Cell Research
Volume volume383
Issue issue2
Publisher Elsevier
Start Page 111556
ISSN 00144827
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Authors. Published by Elsevier Inc.
File Version publisher
PubMed ID 31415758
DOI 10.1016/j.yexcr.2019.111556
Web of Science KeyUT 000488145400017
Related Url isVersionOf https://doi.org/10.1016/j.yexcr.2019.111556
Author Nishida, Keiichiro| Hashizume, Kenzo| Ozawa, Masatsugu| Takeshita, Ayumu| Kaneda, Daisuke| Nakahara, Ryuichi| Nasu, Yoshihisa| Shimamura, Yasunori| Inoue, Hajime| Ozaki, Toshifumi|
Published Date 2017-02
Publication Title Acta Medica Okayama
Volume volume71
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/54824
JaLCDOI 10.18926/AMO/54811
FullText URL 70_6_477.pdf
Author Nishida, Keiichiro| Machida, Takahiro| Horita, Masahiro| Hashizume, Kenzo| Nakahara, Ryuichi| Nasu, Yoshihisa| Ohashi, Hideki| Saiga, Kenta| Ozaki, Toshifumi|
Abstract The metatarsophalangeal (MTP) joints are often and predominantly affected in rheumatoid arthritis. The aim of the current study was to describe surgical techniques of shortening oblique osteotomy for lesser metatarsal bone with screw fixation at the osteotomy site, and to investigate the short-term clinical outcomes of our procedure. Twenty-seven feet (78 toes) of 24 RA patients underwent the shortening oblique osteotomy for the correction of deformity at the lesser MTP joints. The average Japanese Society of Surgery of the Foot (JSSF) standard rating system for the RA foot and ankle scale improved significantly from 59.6 points preoperatively to 88.3 points postoperatively (p<0.001). Twenty-four feet (89 ) were free from metatarsalgia and symptomatic callosities at the lesser MTP joint after surgery. Our present findings showed satisfactory early clinical outcomes of the shortening oblique osteotomy of the metatarsal bone with screw fixation for RA forefoot.
Keywords shortening oblique osteotomy rheumatoid arthritis forefoot screw fixation
Amo Type Original Article
Published Date 2016-12
Publication Title Acta Medica Okayama
Volume volume70
Issue issue6
Publisher Okayama University Medical School
Start Page 477
End Page 483
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2016 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28003673
JaLCDOI 10.18926/AMO/53119
FullText URL 69_1_29.pdf
Author Nakahara, Ryuichi| Nishida, Keiichiro| Hashizume, Kenzo| Harada, Ryouzou| Machida, Takahiro| Horita, Masahiro| Ohtsuka, Aiji| Ozaki, Toshifumi|
Abstract The outcome measures in rheumatology clinical trials (OMERACT) scores are the most mature quantitation system for rheumatoid arthritis (RA) on magnetic resonance imaging (MRI). Direct measuring techniques of synovial volume have been reported with good reproducibility, although few reports have demonstrated the changes of these measures in response to treatment. To assess these clinical responses, we evaluated the correlation of the changes of clinical activity score 28-joints disease activity score (DAS28-CRP) with the changes of OMERACT scores and with synovial volume measurements. Eight RA patients who were treated by biologic agents were examined with MRI of the dominant affected wrist and finger joints before and one year after the treatment. The total OMERACT score was reduced from 48.0 to 41.3, and synovial volume was reduced from 15.4 to 8.8 milliliters. Positive correlations were seen between the changes of DAS28-CRP and the changes of OMERACT synovitis score (r=0.27), OMERACT total score (r=0.43) and synovial volume (r=0.30). Limited to synovium assessment, synovial volume showed a better correlation with DAS28-CRP than the OMERACT synovitis score. On the other hand, the OMERACT total score showed a higher correlation with DAS28-CRP than synovial volume, probably because the OMERACT total score includes scores for bone erosion and bone edema as well.
Keywords magnetic resonance imaging rheumatoid arthritis outcome measures in rheumatology clinical trials scoring system direct volume measuring medical work station
Amo Type Original Article
Published Date 2015-02
Publication Title Acta Medica Okayama
Volume volume69
Issue issue1
Publisher Okayama University Medical School
Start Page 29
End Page 35
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 25703168
Web of Science KeyUT 000349740300003
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/53113
Title Alternative Hospital and clinic cooperation for the treatment of rheumatoid arthritis in Okayama Prefecture, Japan
FullText URL 126_209.pdf
Author Sada, Ken-ei| Nishida, Keiichiro| Yamanaka, Takao| Misaki, Kenta| Wakabayashi, Hiroshi| Shinoda, Junko| Takagi, Toru| Yano, Ryusuke| Nakamura, Akihiko| Nanba, Yoshifumi| Morita, Yoshitaka| Koyama, Yoshinobu| Yamamoto, Keiji| Ezawa, Kazuhiko| Ota, Yusuke| Yoshihara, Yoshiki| Miyoshi, Shinya| Natsumeda, Masamitsu| Usui, Masaaki| Yoshinaga, Yasuhiko| Hayashi, Takashi| Yamamura, Masahiro| Hashizume, Hiroyuki|
Abstract Objective: To survey the current status and problems of cooperation between clinics and hospitals in Okayama Prefecture, Japan for the treatment of rheumatoid arthritis (RA).  Methods: We distributed a questionnaire to 300 of the 983 Okayama Prefecture clinics that had either an internal medicine or orthopedic surgery department, from December 2013 to February 2014. The questionnaire covered practice pattern for RA treatment in clinics, current status of the hospital and clinic cooperation, and acceptance of the biologic therapy.  Results: One hundred clinics responded to the questionnaire. Seventy percent of the clinics reported making referrals to rheumatologists before the initiation of RA treatment, and half of the other 30% of the clinics administered methotrexate as the first-line treatment for RA by their own decision. Sixty-six clinics cooperated with flagship hospitals, conducting medical and laboratory examinations, providing prescriptions, and treating common diseases of patients. These clinics expected the cooperating rheumatologists to follow-up patients every 3 to 6 months and to make the diagnosis, make decisions regarding RA treatment changes, and perform surgery. Seventy-one percent of the clinics responded that cooperation with a hospital is possible even for patients who are administered biologics. As reasons for no cooperation with the flagship hospitals, clinics noted the lack of information about rheumatologists in the area and recent trends in the management of RA.  Conclusion: The current study reported, for the first time, the actual conditions of management of RA in clinics, as well as future problems of hospital and clinic cooperation in Okayama Prefecture.
Keywords 病診連携(hospital and clinic cooperation) 関節リウマチ(rheumatoid arthritis) 生物学的製剤(biologics) メトトレキサート(methotrexate)
Publication Title 岡山医学会雑誌
Published Date 2014-12-01
Volume volume126
Issue issue3
Start Page 209
End Page 215
ISSN 0030-1558
language 日本語
Copyright Holders Copyright (c) 2014 岡山医学会
File Version publisher
DOI 10.4044/joma.126.209
NAID 130004903246
JaLCDOI 10.18926/AMO/51867
FullText URL 67_5_311.pdf
Author Nishida, Keiichiro| Hashizume, Hiroyuki| Matsukawa, Akihiro| Hashizume, Kenzo| Shimamura, Yasunori| Torigoe, Yasuyuki| Ozaki, Toshifumi|
Abstract We report a case of 4th metacarpal head collapse of a 19-year-old healthy man. MRI revealed T1 low and T2 high regions in the collapsed 4th metacarpal head, as well as in the right 3rd and left 4th metacarpal head. Our initial diagnosis was occult compression fracture due to avascular necrosis, known as Dieterich's disease. However, pathological findings of surgically resected right 4th metacarpal head were compatible with transient osteoporosis and metacarpal head fracture followed by active tissue repair. The autologous osteochondral transplants from costchondral junction survived and maintained their size and shape even at 10-year follow-up.
Keywords occult compression fracture metacarpal head avascular necrosis osteochondral autograft
Amo Type Case Report
Published Date 2013-10
Publication Title Acta Medica Okayama
Volume volume67
Issue issue5
Publisher Okayama University Medical School
Start Page 311
End Page 317
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 24145731
Web of Science KeyUT 000325836100005
JaLCDOI 10.18926/AMO/49255
FullText URL 67_1_35.pdf
Author Watanabe, Masutaka| Arita, Seizaburo| Hashizume, Hiroyuki| Honda, Mitsugi| Nishida, Keiichiro| Ozaki, Toshifumi|
Abstract The purpose of this study was to quantitatively evaluate Akahori's preoperative classification of cubital tunnel syndrome. We analyzed the results for 57 elbows that were treated by a simple decompression procedure from 1997 to 2004. The relationship between each item of Akahori's preoperative classification and clinical stage was investigated based on the parameter distribution. We evaluated Akahori's classification system using multiple regression analysis, and investigated the association between the stage and treatment results. The usefulness of the regression equation was evaluated by analysis of variance of the expected and observed scores. In the parameter distribution, each item of Akahori's classification was mostly associated with the stage, but it was difficult to judge the severity of palsy. In the mathematical evaluation, the most effective item in determining the stage was sensory conduction velocity. It was demonstrated that the established regression equation was highly reliable (R=0.922). Akahori's preoperative classification can also be used in postoperative classification, and this classification was correlated with postoperative prognosis. Our results indicate that Akahori's preoperative classification is a suitable system. It is reliable, reproducible and well-correlated with the postoperative prognosis. In addition, the established prediction formula is useful to reduce the diagnostic complexity of Akahori's classification.
Keywords cubital tunnel syndrome ulnar nerve Akahoriʼs classification multiple regression analysis
Amo Type Original Article
Published Date 2013-02
Publication Title Acta Medica Okayama
Volume volume67
Issue issue1
Publisher Okayama University Medical School
Start Page 35
End Page 44
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2013 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 23439507
Web of Science KeyUT 000316829900005
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/50647
JaLCDOI 10.18926/AMO/47262
FullText URL 65_6_369.pdf
Author Terada, Chuji| Yoshida, Aki| Nasu, Yoshihisa| Mori, Shuji| Tomono, Yasuko| Tanaka, Masato| Takahashi, Hideo K.| Nishibori, Masahiro| Ozaki, Toshifumi| Nishida, Keiichiro|
Abstract We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glycation end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the translocation of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.
Keywords HMGB-1 RAGE chondrocyte osteoarthritis cartilage
Amo Type Original Article
Published Date 2011-12
Publication Title Acta Medica Okayama
Volume volume65
Issue issue6
Publisher Okayama University Medical School
Start Page 369
End Page 377
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22189477
Web of Sience KeyUT 000298516900003
Author Shimamura, Yasunori| Inoue, Madoka| Ozawa, Masatugu| Kanazawa, Tomoko| Saitou, Taiichi| Nakahara, Ryuichi| Noda, Tomoyuki| Nishida, Keiichiro| Ozaki, Toshifumi|
Published Date 2011-08-01
Publication Title 岡山医学会雑誌
Volume volume123
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/40012
FullText URL fulltext.pdf
Author Sasaki, Kentaro| Senda, Masuo| Nishida, Keiichiro| Ota, Haruyuki|
Abstract We examined whether the preoperative time required for the Timed "Up and Go" (TUG) test could predict the risk for deep venous thrombosis (DVT) in patients with hip osteoarthritis after total hip arthroplasty (THA). Eighteen patients with DVT diagnosed by venography were selected, and 18 without DVT of the same age and sex and with the same operated side as the DVT group were selected as a control group. We evaluated the 5 preoperative factors that might affect the occurrence of DVT complications, as follows:disease duration, body mass index, serum total cholesterol, subjective pain evaluated by the visual analog scale, and TUG. The JOA hip score (pain, range of motion, walking ability, and daily life) was also evaluated before surgery. As a postoperative factor, we checked the postoperative day when weight-bearing was initiated. As a result, TUG (DVT, 18.4+/-4.0 sec vs. control, 15.0+/-3.2 sec;p0.01) was only significantly different between the 2 groups. The ROC curve revealed that the cut-off point of 15.3 sec in preoperative time for TUG was sensitive (83.3%) and specific (61.1%) for DVT after THA (odds ratio7.0;95% confidence interval, 1.6-30.8). These results suggested that low preoperative ambulatory ability in patients with hip osteoarthritis might be associated with DVT after THA. An improvement in TUG before surgery might contribute to a decrease in the occurrence of DVT after THA.
Keywords preoperative Timed “Up and Go” test deep venous thrombosis total hip arthroplasty hip osteoarthritis
Amo Type Original Article
Published Date 2010-06
Publication Title Acta Medica Okayama
Volume volume64
Issue issue3
Publisher Okayama University Medical School
Start Page 197
End Page 201
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 20596131
Web of Science KeyUT 000279094300006
JaLCDOI 10.18926/AMO/32855
FullText URL fulltext.pdf
Author Shimamura, Yasunori| Nishida, Keiichiro| Imatani, Junya| Noda, Tomoyuki| Hashizume, Hiroyuki| Ohtsuka, Aiji| Ozaki, Toshifumi|
Abstract <p>We biomechanically evaluated the bone fixation rigidity of an ONI plate (Group I) during fixation of experimentally created transcondylar humerus fractures in cadaveric elbows, which are the most frequently observed humeral fractures in the elderly, and compared it with the rigidity achieved by 3 conventional fixation methods:an LCP reconstruction plate 3.5 using a locking mechanism (Group II), a conventional reconstruction plate 3.5 (CRP) with a cannulated cancellous screw (Group III), and a CRP with 2 cannulated cancellous screws (CS) in a crisscross orientation (Group IV). In the axial loading test, the mean failure loads were:Group I, 98.9+/-32.6;Group II, 108.5+/-27.2;Group III, 50.0+/-7.5;and Group IV, 34.5+/-12.2 (N). Group I fixations failed at a significantly higher load than those of Groups III and IV (p0.05). In the extension loading test, the mean failure loads were:Group I, 34.0+/-12.4;Group II, 51.0+/-14.8;Group III, 19.3+/-6.0;and Group IV, 14.7+/-3.1 (N). Group IV fixations showed a significantly lower failure load than those of Group I (p0.05). The fixation rigidities against mechanical loading by the ONI plate and LCP plate were comparable. These results suggested that an ONI system might be superior to the CRP and CS method, and comparable to the LCP method in terms of fixation rigidity for distal humerus fractures.</p>
Keywords distal humerus fracture biomechanics internal fixation elderly
Amo Type Original Article
Published Date 2010-04
Publication Title Acta Medica Okayama
Volume volume64
Issue issue2
Publisher Okayama University Medical School
Start Page 115
End Page 120
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20424666
Web of Science KeyUT 000276996900005
JaLCDOI 10.18926/AMO/31820
FullText URL fulltext.pdf
Author Miyake, Kohei| Nishida, Keiichiro| Kadota, Yasutaka| Yamasaki, Hiroko| Nasu, Tatsuyo| Saitou, Daisuke| Tanabe, Katsuyuki| Sonoda, Hikaru| Sato, Yasufumi| Maeshima, Yohei| Makino, Hirofumi|
Abstract <p>Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immunohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (p0.002, r0.842). In vitro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.</p>
Keywords angiogenesis vasohibin-1 rheumatoid arthritis synovial membrane VEGF
Amo Type Original Article
Published Date 2009-12
Publication Title Acta Medica Okayama
Volume volume63
Issue issue6
Publisher Okayama University Medical School
Start Page 349
End Page 358
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20035291
Web of Science KeyUT 000273145900006
JaLCDOI 10.18926/AMO/31823
FullText URL fulltext.pdf
Author Tanaka, Masato| Nakanishi, Kazuo| Sugimoto, Yoshihisa| Misawa, Haruo| Takigawa, Tomoyuki| Nishida, Keiichiro| Ozaki, Toshifumi|
Abstract <p>Scoliosis is a common clinical manifestation of Rett syndrome, a neurodevelopmental disorder that almost exclusively affects females. The spinal curve in patients with Rett syndrome is typically a long C curve of a neuromuscular type. As the onset of the scoliosis is very early and shows rapid progression, early surgical intervention has been recommended to prevent a life-threatening collapsing spine syndrome. However, there are high perioperative risks in Rett syndrome patients who undergo spinal surgery, such as neurological compromise and respiratory dysfunction due to rigid spinal curve. We herein report the surgical result of treating severe rapid progressive thoracic scoliosis in a 16-year-old girl with Rett syndrome. Posterior segmental pedicle screw fixation was performed from T1 to L3 using a computer-assisted technique. Post-operative radiography demonstrated a good correction of the curve in both the sagittal and coronal alignment. There were no postoperative complications such as neurological compromise. The patient had maintained satisfactory spinal balance as of the 3-year follow-up examination.</p>
Keywords Rett syndrome scoliosis computer navigation-assisted surgery segmental pedicle screw fixation
Amo Type Case Report
Published Date 2009-12
Publication Title Acta Medica Okayama
Volume volume63
Issue issue6
Publisher Okayama University Medical School
Start Page 373
End Page 377
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20035294
Web of Science KeyUT 000273145900009
Author Tetsunaga, Tomonori| Furumatsu, Takayuki| Abe, Nobuhiro| Nishida, Keiichiro| Naruse, Keiji| Ozaki, Toshifumi|
Published Date 2009-09-18
Publication Title Journal of Biomechanics
Volume volume42
Issue issue13
Content Type Journal Article
JaLCDOI 10.18926/AMO/30974
FullText URL fulltext.pdf
Author Ikuma, Hisanori| Abe, Nobuhiro| Uchida, Youichiro| Furumatsu, Takayuki| Fujiwara, Kazuo| Nishida, Keiichiro| Ozaki, Toshifumi|
Abstract <p>Instability of the knee after the medial collateral ligament (MCL) injury is usually assessed with the manual valgus stress test, even though, in recent years, it has become possible to apply magnetic resonance imaging (MRI) to the assessment of the damage of the ligament. The valgus instability of 24 patients (12 isolated injuries and 12 multiple ligament injuries) who suffered MCL injury between 1993 and 1998 was evaluated with the Hughston and Eilers classification, which involves radiographic assessment under manual valgus stress to the injured knees. We developed a novel system for classifying the degree of injury to the MCL by calculating the percentage of injured area based on MRI and investigated the relationship between this novel MRI classification and the magnitude of valgus instability by the Hughston and Eilers classification. There was a significant correlation between the 2 classifications (p=0.0006). On the other hand, the results using other MRI based classification systems, such as the Mink and Deutsch classificaiton and the Petermann classification, were not correlated with the findings by the Hughston and Eilers classification in these cases (p0.05). Since MRI is capable of assessing the injured ligament in clinical practice, this novel classification system would be useful for evaluating the stability of the knee and choosing an appropriate treatment following MCL injury.</p>
Keywords medial collateral ligament magnetic resonance imaging knee instability novel method
Amo Type Original Article
Published Date 2008-06
Publication Title Acta Medica Okayama
Volume volume62
Issue issue3
Publisher Okayama University Medical School
Start Page 185
End Page 191
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18596835
Web of Science KeyUT 000257130300006
JaLCDOI 10.18926/AMO/30956
FullText URL fulltext.pdf
Author Doi, Hideyuki| Nishida, Keiichiro| Yorimitsu, Masanori| Komiyama, Takamitsu| Kadota, Yasutaka| Tetsunaga, Tomonori| Yoshida, Aki| Kubota, Satoshi| Takigawa, Masaharu| Ozaki, Toshifumi|
Abstract <p>Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type II collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1beta expression was also signifi cantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes.</p>
Keywords IL-4 MMP cathepsin B mechanical stress aggrecanase
Amo Type Original Article
Published Date 2008-04
Publication Title Acta Medica Okayama
Volume volume62
Issue issue2
Publisher Okayama University Medical School
Start Page 119
End Page 126
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18464888
Web of Science KeyUT 000255297600008
JaLCDOI 10.18926/AMO/30749
FullText URL fulltext.pdf
Author Komiyama, Takamitsu| Nishida, Keiichiro| Yorimitsu, Masanori| Doi, Hideyuki| Miyazawa, Shinichi| Kitamura, Ai| Yoshida, Aki| Nasu, Yoshihisa| Abe, Nobuhiro| Ozaki, Toshifumi|
Abstract Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p&#60;0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR.
Keywords spontaneous hypertensive rats insulin like growth factor-1 vascular endothelial growth factor ossification disturbance osteonecrosis
Amo Type Article
Published Date 2006-06
Publication Title Acta Medica Okayama
Volume volume60
Issue issue3
Publisher Okayama University Medical School
Start Page 141
End Page 148
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16838042
Web of Science KeyUT 000238503600001