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ID 30956
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Author
Doi, Hideyuki
Nishida, Keiichiro Kaken ID publons researchmap
Yorimitsu, Masanori
Komiyama, Takamitsu
Kadota, Yasutaka
Tetsunaga, Tomonori
Yoshida, Aki
Takigawa, Masaharu Kaken ID publons researchmap
Abstract

Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type II collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1beta expression was also signifi cantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes.

Keywords
IL-4
MMP
cathepsin B
mechanical stress
aggrecanase
Amo Type
Original Article
Published Date
2008-04
Publication Title
Acta Medica Okayama
Volume
volume62
Issue
issue2
Publisher
Okayama University Medical School
Start Page
119
End Page
126
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT