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ID 32287
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Author
Shibahara, Motomi
Nishida, Keiichiro Kaken ID researchmap
Asahara, Hiroshi
Yoshikawa, Takeshi
Mitani, Shigeru
Kondo, Yoichi
Inoue, Hajime
Abstract

We investigated the presence of osteocyte apoptosis in the necrotic trabeculae of the femoral head of spontaneously hypertensive rat (SHR) using the in situ nick end labeling (TUNEL) method and transmission electron microscopy. The occurrence of osteonecrosis and ossification disturbance was significantly higher in SHR compared with Wistar Kyoto (WKY) rats, and Wistar (WT) rats used as control animals (P < 0.01). A high population of TUNEL positive osteocytes was detected mainly in 10- and 15-week-old SHRs. Sectioned examination of the femoral head of SHRs and WKY rats by electron microscopy revealed apoptotic cell appearances such as aggregation of chromatin particles and lipid formation. In contrast, a positive reaction was significantly lower in osteocytes in the femoral heads of WT rats (P < 0.01). Our results indicate that apoptosis forms an important component of the global pathologic process affecting the femoral head of SHR, which leads to osteonecrosis in this region.

Keywords
apoptosis
spontaneously hypertensive rat
osteonecrosis of the femoral head
Amo Type
Article
Published Date
2000-04
Publication Title
Acta Medica Okayama
Volume
volume54
Issue
issue2
Publisher
Okayama University Medical School
Start Page
67
End Page
74
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT