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ID 58554
Author
Masamoto, Michimasa Department of Oral Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mitoh, Yoshihiro Department of Oral Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID researchmap
Kobashi, Motoi Department of Oral Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID researchmap
Shigemura, Noriatsu Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University
Yoshida, Ryusuke Department of Oral Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID researchmap
Abstract
Bitter taste receptors TAS2Rs detect noxious compounds in the oral cavity. Recent heterologous expression studies reported that some compounds function as antagonists for human TAS2Rs. For examples, amino acid derivatives such as γ-aminobutyric acid (GABA) and Nα,Nα-bis(carboxymethyl)-L-Lysine (BCML) blocked responses to quinine mediated by human TAS2R4. Probenecid inhibited responses to phenylthiocarbamide mediated by human TAS2R38. In this study, we investigated the effects of these human bitter receptor antagonists on behavioral lick responses of mice to elucidate whether these compounds also function as bitter taste blockers. In short-term (10 s) lick tests, concentration-dependent lick responses to bitter compounds (quinine-HCl, denatonium and phenylthiourea) were not affected by the addition of GABA or BCML. Probenecid reduced aversive lick responses to denatonium and phenylthiourea but not to quinine-HCl. In addition, taste cell responses to phenylthiourea were inhibited by probenecid. These results suggest some bitter antagonists of human TAS2Rs can work for bitter sense of mouse.
Keywords
bitter coding
bitter inhibitor
gustatory response
species difference
taste perception
Published Date
2020-05-13
Publication Title
Neuroscience Letters
Publisher
Elsevier
Start Page
135041
ISSN
0304-3940
NCID
AA00754925
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
PubMed ID
DOI
Related Url
isVersionOf https://doi.org/10.1016/j.neulet.2020.135041
License
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
Citation
Masamoto M, Mitoh Y, Kobashi M, Shigemura N, Yoshida R. Effects of bitter receptor antagonists on behavioral lick responses of mice [published online ahead of print, 2020 May 12]. Neurosci Lett. 2020;135041. doi:10.1016/j.neulet.2020.135041