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ID 49924
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Author
Shimoura, Yasumasa
Sato, Yasuharu Kakenhi
Takata, Katsuyoshi Kakenhi
Orita, Yorihisa Kakenhi
Nakamura, Satoko
Mano, Shyouhe
Yoshino, Tadashi
Abstract
Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.
Keywords
Cytology
Immunocytochemical
double staining
Immunoglobulin light-chain restriction
Lymph node
B cell lymphoma
Flow cytometry
Published Date
2013
Publication Title
Acta Cytologica
Volume
volume57
Issue
issue1
Start Page
84
End Page
90
ISSN
0001-5547
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1159/000342475
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/49730
language
英語
Copyright Holders
Copyright 2012 S. Karger AG, Basel
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author
Refereed
True
DOI
Web of Science KeyUT