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ID 52856
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Author
Hanada, Hideki
Yamamoto, Masanao
Kashiwagi, Keiko
Katsu, Kenjiro
Utsumi, Toshihiko
Kashiwagi, Akihiko
Sasaki, Junzo
Inoue, Masayasu
Utsumi, Kozo
Abstract
Mitochondrial membrane permeability transition (MPT) plays a crucial role in apoptotic tail shortening during anuran metamor phosis. L-carnitine is known to shuttle free fatty acids (FFAs) from the cytosol into mitochondria matrix for -oxidation and energy production, and in a previous study we found that treatment with L-carnitine suppresses 3, 3', 5-triiodothyronine (T3) and FFA-induced MPT by reducing the level of FFAs. In the present study we focus on acetyl-L-carnitine, which is also involved in fatty acid oxidation, to determine its effect on T3-induced tail regression in Rana rugosa tadpoles and spontaneous tail regression in Xenopus laevis tadpoles. The ladder-like DNA profile and increases in caspase-3 and caspase-9 indicative of apoptosis in the tails of T3-treated tadpoles were found to be suppressed by the addition of acetyl-L-carnitine. Likewise, acetyl-L-carnitine was found to inhibit thyroid hormone regulated spontaneous metamorphosis in X. laevis tadpoles, accompanied by decreases in caspase and phospholipase A2 activity, as well as non-ladder-like DNA profiles. These findings support our previous conclusion that elevated levels of FFAs initiate MPT and activate the signaling pathway controlling apoptotic cell death in tadpole tails during anuran metamorphosis.
Published Date
2013-02
Publication Title
Hereditas
Volume
volume150
Issue
issue1
Start Page
1
End Page
9
ISSN
0018-0661
Content Type
Journal Article
language
英語
Copyright Holders
© 2013 The Authors.
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publisher
Refereed
True
DOI
Web of Sience KeyUT