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ID 31858
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Author
Ogawa, Tomoyuki
Ichikawa, Tomotsugu Kaken ID publons researchmap
Arimitsu, Seiji
Onoda, Keisuke
Tomizawa, Kazuhito
Abstract

Many studies have shown that a motif of 11 consecutive arginines (11R) is one of the most effective protein transduction domains (PTD) for introducing proteins into the cell membrane. By conjugating this "11R", all sorts of proteins can effectively and harmlessly be transferred into any kind of cell. We therefore examined the transduction efficiency of 11R in cerebral arteries and obtained results showing that 11R fused enhanced green fluorescent protein (11R-EGFP) immediately and effectively penetrated all layers of the rat basilar artery (BA), especially the tunica media. This method provides a revolutionary approach to cerebral arteries and ours is the first study to demonstrate the successful transductionof a PTD fused protein into the cerebral arteries. In this review, we present an outline of our studies and other key studies related to cerebral vasospasm and 11R, problems to be overcome, and predictions regarding future use of the 11R protein transduction method for cerebral vasospasm (CV).

Keywords
cerebral vasospasm
11 consecutive arginines (11R)
enhanced green fluorescent protein (EGFP)
Amo Type
Review
Published Date
2009-02
Publication Title
Acta Medica Okayama
Volume
volume63
Issue
issue1
Publisher
Okayama University Medical School
Start Page
1
End Page
7
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT