JaLCDOI 10.18926/AMO/47011
FullText URL 65_5_299.pdf
Author Itani, Miki| Yamamoto, Yuji| Doi, Yusuke| Miyaishi, Satoru|
Abstract Postmortem degradation of DNA was quantitatively estimated. Brain, liver, kidney and muscle samples were obtained from sacrificed rats left at 20℃ or 4℃. The quantity of DNA was measured by real-time PCR using a primer set for a sequence in the Rsrc 1 gene. When the quantity of amplified DNA using 10ng Human Genomic DNA was defined as 100 RFU, the quantities in the brain, liver, kidney and skeletal muscle (each 2μg of dry weight) on the day of sacrifice were 253±11, 338±22, 556±14 and 531±12 Relative Fluorescence Units (RFU), respectively (mean±S.E., n=5). The quantity of amplified DNA decreased to below 10 RFU in 1-3 weeks in the liver, kidney and skeletal muscle at 20℃, while that in the brain was more than 10 RFU for six weeks, demonstrating the usefulness of the brain as a sample for DNA analysis of decaying corpses. It was suggested that quantifying the amplified DNA in the brain at 20℃ and in the liver at 4℃ as well as the ratio of the quantity of amplified DNA in the liver to the brain at 4℃ might be useful for diagnosing time of death. This study provides the first quantitative analysis of the postmortem progress of DNA degradation in the corpse.
Keywords DNA degradation postmortem interval personal identification
Amo Type Original Article
Published Date 2011-10
Publication Title Acta Medica Okayama
Volume volume65
Issue issue5
Publisher Okayama University Medical School
Start Page 299
End Page 306
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22037266
Web of Sience KeyUT 000296116400003
JaLCDOI 10.18926/AMO/46847
FullText URL 65_4_225.pdf
Author Miura, Masanobu| Naka, Toru| Miyaishi, Satoru|
Abstract Postmortem changes in myoglobin concentrations in blood and organs were investigated using an enzyme immunoassay by animal experiments in combination with immunohistochemical staining of human cases. Blood myoglobin concentrations were found to increase drastically within a very short time after death. Those in striated muscle, however, did not change by day 14 postmortem. Myoglobin content in the liver and kidney increased slightly by day 5 postmortem, and more obviously by day 7 or later. However, almost no change was observed by day 5 in the kidney when the renal artery and vein had been ligated just after death. In the thyroid gland and the lung, the myoglobin content markedly increased by day 7 postmortem, with the logarithmical values rising nearly linearly as the time after death passed. In the thyroid gland, concentrations reached the level of the striated muscle. The mechanisms of postmortem myoglobin increase in organs are thought to be direct diffusion from the striated muscle and/or distribution through the blood. To estimate the postmortem interval, the determination of myoglobin content in the thyroid gland or the lung appears to be useful.
Keywords myoglobin postmortem diffusion postmortem distribution postmortem interval
Amo Type Original Article
Published Date 2011-08
Publication Title Acta Medica Okayama
Volume volume65
Issue issue4
Publisher Okayama University Medical School
Start Page 225
End Page 230
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 21860528
Web of Science KeyUT 000294236700002
JaLCDOI 10.18926/AMO/45270
FullText URL 65_2_113.pdf
Author Morikawa, Toshio| Yamamoto, Yuji| Miyaishi, Satoru|
Abstract We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male:female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.
Keywords sex determination SRY (sex-determining region Y) multiplex PCR forensic casework
Amo Type Original Article
Published Date 2011-04
Publication Title Acta Medica Okayama
Volume volume65
Issue issue2
Publisher Okayama University Medical School
Start Page 113
End Page 122
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 21519369
Web of Science KeyUT 000289818800007
JaLCDOI 10.18926/AMO/32819
FullText URL fulltext.pdf
Author Okamoto, Osamu| Yamamoto, Yuji| Inagaki, Sachiyo| Yoshitome, Kei| ishikawa, Takaki| Imabayashi, Kiyomi| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>Allele and genotype frequencies for 15 short tandem repeat (STR) polymorphisms--D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA--in a Japanese population were estimated. No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for any of the systems studied. Between 2 new pentanucleotide STR loci, Penta E and Penta D, for which there is only limited data regarding the allelic distribution in Japanese, the Penta E locus was found to be highly polymorphic and exhibited a tri- or tetra-modal distribution pattern having allelic peaks with 5, 11, 15 and 20 repeats. The distribution was significantly different from that of the other ethnic groups. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity values (H), polymorphism information content (PIC), power of discrimination (PD), matching probability (pM), power of exclusion (PE), and typical paternity index (PI), were calculated for the loci. These parameters indicated the usefulness of the loci in forensic personal identification and paternity testing among Japanese. The systems Penta E, FGA, D18S51 and D8S1179 were the most informative. This method was successfully applied to forensic personal identification and paternity testing among Japanese, thereby confirming its efficacy for forensic practice.</p>
Keywords population data DNA typing short tandem repests personal identification paternity testing
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 59
End Page 71
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866745
Web of Sience KeyUT 000182520400003
JaLCDOI 10.18926/AMO/32818
FullText URL fulltext.pdf
Author Ishikawa, Takaki| Tachibana, Toshiaki| Ishikawa, Hiroshi| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>Healthy subjects 40 years old were used as controls in a study of stellate cells (S-100 protein-containing cells, or S-100 cells) in subjects with chronic alcoholism and fatty liver or fatty cirrhosis. S-100 cells were sparsely found in the adenohypophysis of control subjects, and these cells sometimes formed small clusters. However, in chronic alcoholics with fatty liver or fatty cirrhosis, the number of stellate cells in the anterior pituitary tended to be 17 times higher than it was in the control group. No increase in the number of S-100 positive cells that constitute the large and small follicles in the intermediate pituitary. The physiological function of the S-100 protein has not yet been identified. The fact that an increase in prolactin-secreting and growth hormone-secreting cells, as well as a decrease in gonadotrophs were observed in the hypophysis of alcoholics suggests that the function of stellate cells may be closely related to these phenomena. Our results also imply that the stellate cells found in the anterior and intermediate pituitary differ in function although they both produce S-100 proteins.</p>
Keywords S-100 protein pituitary alcoholism
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 53
End Page 58
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866744
Web of Science KeyUT 000182520400002
JaLCDOI 10.18926/AMO/32816
FullText URL fulltext.pdf
Author Ishikawa, Takaki| Miyaishi, Satoru| Tachibana, Toshiaki| Yamamoto, Yuji| Ishizu, Hideo|
Abstract <p>In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicles also tended to enlarge with age. Statistical analysis showed that a high correlation existed between age and the number or the size of the mixed cell-follicles formed by various adenohypophyseal cells. In addition, when the proportions of the different cell types that formed the follicles were examined, sex differences were observed with aging for the GH cells, the PRL cells, and the gonadotroph (GTH) cells, while no changes were observed with aging in both men and women for the ACTH cells and TSH cells. These results indicate that the number, size, and ratio of each component cell of follicles in the anterior pituitary are adequately applicable for the purpose of age estimation in routine forensic medicine.</p>
Keywords mixed cell-follicle human anterior pituitary age estimation
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 83
End Page 89
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866748
Web of Science KeyUT 000182520400006
JaLCDOI 10.18926/AMO/32309
FullText URL fulltext.pdf
Author Murakami, Hiroki| Ymamamoto, Yuji| Yoshitome, Kei| Ono, Toshiaki| Okamoto, Osamu| Shigeta, Yoshiaki| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>In this study, sex determination using polymerase chain reaction (PCR) on tooth material was evaluated from the viewpoint of forensic medicine. The sensitivity of PCR for detection of the Y chromosome-specific alphoid repeat sequence and the X chromosome-specific alphoid repeat sequence was 0.5 pg of genomic DNA. Sex could be determined by PCR of DNA extracted from the pulp of 16 freshly extracted permanent teeth and dentine including the surface of the pulp cavity of 6 freshly extracted milk teeth. Sex could be determined using the pulp in all 20 teeth (10 male and 10 female) preserved at room temperature for 22 years. For the pulp of teeth stored in sea water, the sex could be determined in all 8 teeth immersed for 1 week and in 5 of 6 teeth immersed for 4 weeks. In the remaining 1 tooth, in which sex determination based on the pulp failed, the sex could be determined correctly when DNA extracted from the tooth hard tissue was examined. For teeth stored in soil, the sex could be determined accurately in all 8 teeth buried for 1 week, 7 of 8 teeth buried for 4 weeks, and in all 6 teeth buried for 8 weeks. When teeth were heated for 30 min, sex determination from the pulp was possible in all teeth heated to 100, 150, and 200 degrees C, and even in some teeth heated to 250 degrees C. When this method was applied to actual forensic cases, the sex of a mummified body estimated to have been discovered half a year to 1 year after death could be determined readily by examination of the dental pulp. In the skeletons of 2 bodies placed under water for approximately 1 year and approximately 11 years and 7 months, pulp tissues had been dissolved and lost, but sex determination was possible using DNA extracted from hard dental tissues. These results indicate that this method is useful in forensic practices for sex determination based on teeth samples.</p>
Keywords personal identification sex determination tooth deoxyribonucleic acid (DNA). polymerase chain reaction
Amo Type Article
Published Date 2000-02
Publication Title Acta Medica Okayama
Volume volume54
Issue issue1
Publisher Okayama University Medical School
Start Page 21
End Page 32
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10709619
Web of Science KeyUT 000085526000004
JaLCDOI 10.18926/AMO/32024
FullText URL fulltext.pdf
Author Ono, Toshiaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Ishikawa, Takaki| Ishizu, Hideo|
Abstract <p>We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.&#60;/P&#62;</p>
Keywords species identification myoglobin polymerase chain reaction
Amo Type Article
Published Date 2001-06
Publication Title Acta Medica Okayama
Volume volume55
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 184
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11434430
Web of Science KeyUT 000169512600004
JaLCDOI 10.18926/AMO/31971
FullText URL fulltext.pdf
Author Imabayashi, Kiyomi| Yamamoto, Yuji| Inagaki, Sachiyo| Doi, Yusuke| Yoshitome, Kei| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.</p>
Keywords HLA-DRB1 genotyping group specific primer single nucleotide polymorphism multiplex primer extension reactions application to mixed samples
Amo Type Article
Published Date 2005-10
Publication Title Acta Medica Okayama
Volume volume59
Issue issue5
Publisher Okayama University Medical School
Start Page 179
End Page 194
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16286957
Web of Science KeyUT 000232835600002
JaLCDOI 10.18926/AMO/31815
FullText URL fulltext.pdf
Author Kamizato, Eigo| Yoshitome, Kei| Yamamoto, Yuji| Iwase, Toshihide| Tsuda, Toshihide| Miyaishi, Satoru| Doi, Hiroyuki|
Abstract <p>The annual number of suicides in Japan increased sharply in 1998, and since that time it has consistently exceeded 30,000 per year. In this study, we analyze a database of personal and background characteristics of 824 cases (605 men, 219 women) who completed suicide in Okayama Prefecture in 2002 and 2003. The data were obtained with cooperation from the police. Using the methodologies in a previous European study as a model, we classified the suicide methods into 8 categories. To examine the generational and regional differences in the choice of methods, we stratified the sample into 4 age groups (&#60;-24, 2544, 4564, and &#62;-65) and 2 regional groups (Okayama/Kurashiki vs. other areas). Our results on gender differences in 7 of the suicide methods were mostly similar to the European data. However, our data showed a remarkably higher proportionate male-to-female mortality ratio for poisoning by other substances (ICD-10, X65-X69 codes) (1.83, 1.15-2.92). In terms of generational differences in the choice of suicide methods, the Mantel-Haenszel test of homogeneity was significant for most of the categories in our study, suggesting an impact of age on how people commit suicide. There were no remarkable regional differences in our sample. An epidemic curve for suicides via carbon monoxide poisoning using charcoal briquets revealed a trend of time clustering not observed in the other 6 means. The database constructed and used in this study contains richer information than conventional death statistics and is expected to provide helpful knowledge and insights for future epidemiological studies.</p>
Keywords suicide methods gender-specific legal medicine cluster suicide
Amo Type Original Article
Published Date 2009-08
Publication Title Acta Medica Okayama
Volume volume63
Issue issue4
Publisher Okayama University Medical School
Start Page 177
End Page 186
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19727202
Web of Science KeyUT 000269228400003
JaLCDOI 10.18926/AMO/31726
FullText URL fulltext.pdf
Author Yoshitome, Kei| Ishikawa, Takaki| Inagaki, Sachiyo| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.</p>
Keywords asphyxia suffocation helium advertising balloon atmosphere lacking in oxygen
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 55
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873946
Web of Science KeyUT 000174031300010
JaLCDOI 10.18926/AMO/31707
FullText URL fulltext.pdf
Author Shigeta, Yoshiaki| Yamamoto, Yuji| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104-156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evaluated using various forensic samples. The typing results from 70 DNA samples using the new primer pair and the original primer pair were completely identical. One hundred twenty-five amplified fragments from D12S391 alleles were sized correctly within +/- 0.25 bp of the D12S391 allelic ladder. A rare allele, 19.3, previously found only in Caucasians, was found for the first time in a Japanese subject, and it was clearly distinguished from allele 20 by the CE analysis. This detection system was sensitive and could detect D12S391 types from 16 pg of genomic DNA, and from a minor component at a ratio of 1:10 in mixed samples. This system was more useful for the analysis of degraded DNA than was the method using the original primer pair, and could detect D12S391 types from bloodstains that had been stored for 26 years. In addition, the specificity of the method was demonstrated using nonhuman DNA.</p>
Keywords short tandem repeats D12S391 forensic application capillary electrophoresis
Amo Type Article
Published Date 2002-10
Publication Title Acta Medica Okayama
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 229
End Page 236
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12530506
Web of Science KeyUT 000178668100003
JaLCDOI 10.18926/AMO/31305
FullText URL fulltext.pdf
Author Inoue, Seiichi| Yamamoto, Yuji| Okamoto, Osamu| Murakami, Hiroki| Miyaishi, Satoru| Isizu, Hideo|
Abstract <p>A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the typing of HLA-DRB1 alleles. By this method, HLA-DRB1 typing was possible from 10 pg of genomic DNA extracted from lymphocytes and from 0.5 microliter of 1,000 times diluted blood without DNA extraction. HLA-DRB1 alleles could be typed from a 2-mm long bloodstained cotton thread prepared from 10 times diluted blood and from a 2-mm thread of whole blood bloodstains stored at room temperature for 2 years. From the mixture of blood of two individuals with different genotypes, DRB1 alleles of the minor component were detected down to 1/1,000 of the major component. This semi-nested PCR-RFLP method is useful for HLA-DRB1 typing from extremely small amounts of DNA and from mixed samples.</p>
Keywords polymorphism HLA-DRB1 polymerase chain reaction dsmi-nested PCR restricton fragment length polymotphism
Amo Type Article
Published Date 1998-12
Publication Title Acta Medica Okayama
Volume volume52
Issue issue6
Publisher Okayama University Medical School
Start Page 289
End Page 296
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9876765
Web of Science KeyUT 000077707300002
JaLCDOI 10.18926/AMO/31301
FullText URL fulltext.pdf
Author Yano, Akemi| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.</p>
Keywords DNA polymorphism haptoglobin polymerase chain reaction allele-specific amplification personal identification
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 173
End Page 181
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781267
Web of Science KeyUT 000075623600001
JaLCDOI 10.18926/AMO/30747
FullText URL fulltext.pdf
Author Redsch, Oliver| Miyaishi, Satoru| Heinemann, Axel| Fiedler, Georg| Puschel, Klaus| Yamamoto, Hideki| Ishizu, Hideo|
Abstract The authors designed a questionnaire to investigate the differences in German and Japanese general practitioners? (GP) awareness of suicide and attitudes toward patients with suicidal ideation in their respective societies. The purpose of this study was to obtain insights leading to a better means of suicide prevention in primary care in Japan. The background for conducting the study was declining suicides in the past 20 years and the lower suicide rate in Germany compared with the present situation in Japan, where the number of suicides has in recent years continued to exceed 30,000, resulting in a suicide rate approximately 2 times higher than that in Germany. The questionnaire was randomly mailed to GPs in Okayama-Prefecture (western Japan) and Hamburg-State (northern Germany) and was collected in the same way. The patterns of answers were compared between the 2 countries, and the differences were statistically analyzed. Japanese GPs seem to have a lower will to prevent suicide in daily practice compared to German GPs and a great lack of knowledge about treatment of suicidal patients. These observations suggest that improving GPs? interest in the problem of suicide and providing training programs for the treatment of patients with suicidal intentions might be a means of achieving better suicide prevention in Japan.
Keywords suicide prevention general practitioner Japan Germany
Amo Type Article
Published Date 2006-06
Publication Title Acta Medica Okayama
Volume volume60
Issue issue3
Publisher Okayama University Medical School
Start Page 159
End Page 165
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16838044
Web of Science KeyUT 000238503600003
Author Nanikawa, Ryo| Hashimoto, Yoshiaki| Moriya, Fumio| Miyaishi, Satoru| Nakai, Miyoko| Yang, Xu| Guo, Chun Gang|
Published Date 1990-02
Publication Title 岡山医学会雑誌
Volume volume102
Issue issue1-2
Content Type Journal Article
Author Miyaishi, Satoru| Kitao, Takashi| Moriya, Fumio| Yamamoto, Yuji| Ishizu, Hideo| Ishii, Hiroyuki|
Published Date 1991-10
Publication Title 岡山医学会雑誌
Volume volume103
Issue issue9-10
Content Type Journal Article
Author Ishizu, Hideo| Miyaishi, Satoru| Yamamoto, Yuji| Takata, Shingo|
Published Date 1993-10
Publication Title 岡山医学会雑誌
Volume volume105
Issue issue9-10
Content Type Journal Article
Author Ishikawa, Takaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Inagaki, Sachiyo| Okamura, Michihiko| Ishizu, Hideo|
Published Date 2001-12-31
Publication Title 岡山医学会雑誌
Volume volume113
Issue issue3
Content Type Journal Article
Author Ishikawa, Takaki| Miyaishi, Satoru| Doi, Yusuke| Takata, Tomoyo| Imabayashi, Kiyomi| Inagaki, Sachiyo| Yoshitome, Kei| Yamamoto, Yuji| Ishizu, Hideo|
Published Date 2003-01-31
Publication Title 岡山医学会雑誌
Volume volume114
Issue issue3
Content Type Journal Article