Author Tada, Kotaro| Hamada, Toshihisa| Asagoe, Kenji| Umemura, Hiroshi| Mizuno-Ikeda, Kazuko| Aoyama, Yumi| Otsuka, Masaki| Yamasaki, Osamu| Iwatsuki, Keiji|
Published Date 2014-11
Publication Title European Journal of Dermatology
Volume volume24
Issue issue6
Content Type Journal Article
JaLCDOI 10.18926/AMO/53118
FullText URL 69_1_17.pdf
Author Suzuki, Norihiro| Takata, Minoru| Shirafuji, Yoshinori| Otsuka, Masaki| Yamasaki, Osamu| Asagoe, Kenji| Hatta, Naohito| Iwatsuki, Keiji|
Abstract Sentinel lymph node (SLN) biopsies have widely been used for the detection of occult LN metastasis of malignant melanoma (MM). In addition to conventional biomarkers, we assessed the diagnostic and prognostic significance of melanoma-initiating cell (MIC) markers in SLNs of MM. We examined the expressions of gp100, MART-1 and tyrosinase mRNA for routine diagnosis and those of ABCB5, CD133, nestin, KDM5B, NGFR and RANK mRNA as MIC markers. The presence of micrometastasis was confirmed immunohistochemically using antibodies to S-100, HMB-45, MART-1, and tyrosinase. Discordance between immunohistochemical and molecular data was observed in 14 of 70 (20.0%) patients, among whom five (7.1%) were positive for only molecular markers;two of these five patients tested positive for micrometastasis by repeated immunohistochemical stainings. The quantitative expression levels of gp100, MART-1, and tyrosinase mRNA were significantly higher in the metastatic LNs;the cut-off values remain to be elucidated. ABCB5 mRNA expression was detected more frequently in the metastatic SLNs (p<0.05) and in the group of patients with recurrence. To make a definite diagnosis of metastasis, we still need a combination of immunohistochemical and molecular probes. ABCB5 might be a suitable molecular marker for the detection of melanoma-initiating cells in SLNs.
Keywords melanoma cancer-initiating cell sentinel lymph node ABCB5
Amo Type Original Article
Published Date 2015-02
Publication Title Acta Medica Okayama
Volume volume69
Issue issue1
Publisher Okayama University Medical School
Start Page 17
End Page 27
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 25703167
Web of Science KeyUT 000349740300002
Related Url
Author Otsuka, Masaki| Asagoe, Kenji| Iwatsuki, Keiji|
Published Date 2008-08-01
Publication Title 岡山医学会雑誌
Volume volume120
Issue issue2
Content Type Journal Article