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ID 30431
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Author
Yamasaki, Yasushi
Makino, Hirofumi
Hironaka, Kazue
Hayashi, Yoshikazu
Ota, Zensuke
Abstract

We demonstrated the ultrastructure of rat glomerular basement membrane (GBM) by ultra-high resolution scanning electron microscopy. GBM prepared by sonication methods and conductive-staining could be observed without metal coating at magnifications as high as 400,000 times. The GBM showed an irregular meshwork structure composed of various strands and pores. The width of the strands ranged from 6 to 15 nm, and the diameter of pores ranged from 6 to 50 nm. The present study confirmed our molecular sieve theory of the basement membrane.

Keywords
glomerular basement membrance
ultrastructure
ultra-high resolution scanning electron microscopy
conductive staining
Amo Type
Article
Published Date
1990-12
Publication Title
Acta Medica Okayama
Volume
volume44
Issue
issue6
Publisher
Okayama University Medical School
Start Page
333
End Page
335
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT