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ID 30764
JaLCDOI
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Author
Ino, Hideo
Hayashi, Kazuhiko
Teramoto, Norihiro
Koirala, Tirtha Raj
Chen, Hong-Li
Akagi, Tadaastu
Abstract

A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.

Keywords
Epstein-Barr virus
HVMF 1
lymphoma
?monkey cell line
PCR
Amo Type
Article
Published Date
1997-08
Publication Title
Acta Medica Okayama
Volume
volume51
Issue
issue4
Publisher
Okayama University Medical School
Start Page
207
End Page
212
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT