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ID 31716
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Author
Alam, Shahjalal S.
Nakamura, Takashi
Naganuma, Atsushi
Nozaki, Akito
Shimomura, Hiroyuki
Abstract

We have shown that highly proofreading DNA polymerase is required for the polymerase chain reaction in the genetic analysis of hepatitis C virus (HCV). To clarify the status of HCV quasispecies in hepatic tissue using proofreading DNA polymerase, we performed a genetic analysis of the HCV core protein-encoding region in cancerous and noncancerous lesions derived from 4 patients with hepatocellular carcinoma. In contrast to the previously published data, we observed neither deletions nor stop codons in the analyzed region and no significant difference in the complexity of HCV quasispecies between cancerous and noncancerous lesions. This result suggests that the HCV core gene is never structurally defective in hepatic tissues, including cancerous lesions. However, in 3 of the patients, the consensus HCV species differed between cancerous and noncancerous lesions, suggesting that the predominant replicating HCV species differs between these 2 types of lesions. Moreover, during the course of the study, we obtained several interesting variants possessing a substitution at codon 9 of the core gene, whose substitution has been shown to induce the production of the F protein synthesized by a - 2/+1 ribosomal frameshift.

Keywords
hepatitis C virus
core gene
hepatocellular carcinoma
quasispecies
proofreading DNA polymerase
Amo Type
Article
Published Date
2002-06
Publication Title
Acta Medica Okayama
Volume
volume56
Issue
issue3
Publisher
Okayama University Medical School
Start Page
141
End Page
147
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT