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ID 55280
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Hisano, Hiroshi Institute of Plant Science and Resources, Okayama University
Meints, Brigid Department Crop and Soil Sciences, Washington State University
Moscou, Matthew J. The Sainsbury Laboratory
Cistue, Luis Department Genetica y Produccion Vegetal
Echávarri, Begoña
Sato, Kazuhiro Institute of Plant Science and Resources, Okayama University Kakenhi
Hayes, Patrick M. Department Crop and Soil Science, Oregon State University
Abstract
Key message: The genetic substitution of transformation amenability alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. Abstract: Barley (Hordeum vulgare) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from ‘Haruna Nijo’ × ‘Golden Promise,’ as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross ‘Golden Promise’ × ‘Full Pint.’ Based on SNP genotype, we selected lines having ‘Golden Promise’ alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to ‘Golden Promise.’ The results validate that the genetic substitution of TFA alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
Keywords
Agrobacterium tumefaciens
Doubled haploid
Hordeum vulgare (barley)
Single nucleotide polymorphism
Transformation
Published Date
2017-04
Publication Title
Plant Cell Reports
Volume
volume36
Issue
issue4
Publisher
Springer
Start Page
611
End Page
620
ISSN
0721-7714
NCID
AA10635864
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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isVersionOf https://doi.org/10.1007/s00299-017-2107-2