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ID 49094
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Author
Mayer, Klaus F. X.
Martis, Mihaela
Hedley, Pete E.
Simkova, Hana
Liu, Hui
Morris, Jenny A.
Steuernagel, Burkhard
Taudien, Stefan
Roessner, Stephan
Gundlach, Heidrun
Kubalakova, Marie
Suchankova, Pavla
Murat, Florent
Felder, Marius
Nussbaumer, Thomas
Graner, Andreas
Salse, Jerome
Endo, Takashi
Sakai, Hiroaki
Tanaka, Tsuyoshi
Itoh, Takeshi
Platzer, Matthias
Matsumoto, Takashi
Scholz, Uwe
Dolezel, Jaroslav
Waugh, Robbie
Stein, Nils
Abstract
We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign; similar to 86% of the estimated; similar to 32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
Published Date
2011-04
Publication Title
The Plant Cell
Volume
volume23
Issue
issue4
Start Page
1249
End Page
1263
ISSN
1040-4651
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1105/tpc.110.082537
language
英語
Copyright Holders
© 2011 American Society of Plant Biologists
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Refereed
True
DOI
Web of Science KeyUT