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ID 49093
FullText URL
Author
Matsumoto, Takashi
Tanaka, Tsuyoshi
Sakai, Hiroaki
Amano, Naoki
Kanamori, Hiroyuki
Kurita, Kanako
Kikuta, Ari
Kamiya, Kozue
Yamamoto, Mayu
Ikawa, Hiroshi
Fujii, Nobuyuki
Hori, Kiyosumi
Itoh, Takeshi
Abstract
Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
Published Date
2011-05
Publication Title
Plant Physiology
Volume
volume156
Issue
issue1
Start Page
20
End Page
28
ISSN
0032-0889
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1104/pp.110.171579
language
英語
File Version
publisher
Refereed
True
DOI
Web of Science KeyUT