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ID 60542
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Author
Abe, Fumitaka Division of Basic Research, Institute of Crop Science, NARO
Ishida, Yuji Plant Innovation Center, Japan Tobacco Inc.
Hisano, Hiroshi Institute of Plant Science and Resources, Okayama University ORCID Kaken ID researchmap
Endo, Masaki Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Komari, Toshihiko Plant Innovation Center, Japan Tobacco Inc.
Toki, Seiichi Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Sato, Kazuhiro Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Abstract
Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used for the analysis of gene function and crop improvement. For complete details on the use and execution of this protocol, please refer to Abe et al. (2019).
Published Date
2020-06-13
Publication Title
STAR Protocols
Volume
volume1
Issue
issue2
Publisher
Cell Press
Start Page
100053
ISSN
2666-1667
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 The Author(s).
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DOI
Related Url
isVersionOf https://doi.org/10.1016/j.xpro.2020.100053
License
https://creativecommons.org/licenses/by/4.0/
Open Access (Publisher)
OA
Open Archive (publisher)
Non-OpenArchive