Author 野口 洋文| 松下 正之| Kobayashi, Naoya| Susan Bonner-Weir| 松井 秀樹| 田中 紀章| 田中 紘一| 松本 慎一|
Published Date 2005-09-01
Publication Title 岡山医学会雑誌
Volume volume117
Issue issue2
Content Type Journal Article
FullText URL 118_205.pdf
Author 道上 宏之| 富澤 一仁| 魏 范研| 松下 正之| 陸 雲飛| 市川 智継| 田宮 隆| 松井 秀樹| 伊達 勲|
Keywords プロテインセラピー 悪性脳腫瘍 p 53 エンドソーム 蛋白導入ドメイン
Publication Title 岡山医学会雑誌
Published Date 2007-01-04
Volume volume118
Issue issue3
Start Page 205
End Page 208
ISSN 00301558
language 日本語
Copyright Holders Copyright© 岡山医学会
File Version publisher
DOI 10.4044/joma1947.118.3_205
NAID 10018454083
Author 松井 秀樹|
Published Date 1982-03-31
Publication Title
Content Type Thesis or Dissertation
Author Matsui, Hideki|
Published Date 1982-04-30
Publication Title 岡山医学会雑誌
Volume volume94
Issue issue3-4
Content Type Journal Article
Title Alternative The 10th Congress on Neutron Capture Therapy
FullText URL 126_73.pdf
Author Matsui, Hideki|
Publication Title 岡山医学会雑誌
Published Date 2014-04-01
Volume volume126
Issue issue1
Start Page 73
End Page 74
ISSN 0030-1558
Related Url http://www.okayama-u.ac.jp/user/oma/
language 日本語
Copyright Holders Copyright (c) 2014 岡山医学会
File Version publisher
DOI 10.4044/joma.126.73
Author Hayashi, Keiichiro| Ueshima, Satoshi| Ouchida, Mamoru| Mashimo, Tomoji| Nishiki, Teiichi| Sendo, Toshiaki| Serikawa, Tadao| Matsui, Hideki| Ohmori, Iori|
Published Date 2011-05
Publication Title Epilepsia
Volume volume52
Issue issue5
Content Type Journal Article
JaLCDOI 10.18926/AMO/43824
FullText URL 65_1_1.pdf
Author Han, Xiao-Jian| Tomizawa, Kazuhito| Fujimura, Atsushi| Ohmori, Iori| Nishiki, Tei-ichi| Matsushita, Masayuki| Matsui, Hideki|
Abstract Mitochondria are important cellular organelles in most metabolic processes and have a highly dynamic nature, undergoing frequent fission and fusion. The dynamic balance between fission and fusion plays critical roles in mitochondrial functions. In recent studies, several large GTPases have been identified as key molecular factors in mitochondrial fission and fusion. Moreover, the posttranslational modifications of these large GTPases, including phosphorylation, ubiquitination and SUMOylation, have been shown to be involved in the regulation of mitochondrial dynamics. Neurons are particularly sensitive and vulnerable to any abnormalities in mitochondrial dynamics, due to their large energy demand and long extended processes. Emerging evidences have thus indicated a strong linkage between mitochondria and neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease and Huntington's disease. In this review, we will describe the regulation of mitochondrial dynamics and its role in neurodegenerative diseases.
Keywords mitochondria phosphorylation ubiquitination SUMOylation neurodegeneration
Amo Type Review
Published Date 2011-02
Publication Title Acta Medica Okayama
Volume volume65
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 10
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 21339790
Web of Science KeyUT 000287620500001
FullText URL Epilepsia_49_3_521.pdf
Author Ohmori, Iori| Ouchida, Mamoru| Kobayashi, Katsuhiro| Jitsumori, Yoshimi| Inoue, Takushi| Shimizu, Kenji| Matsui, Hideki| Ohtsuka, Yoko| Maegaki, Yoshihiro|
Keywords Rasmussen encephalitis SCN1A genetic-environmental interaction
Published Date 2007-11-21
Publication Title Epilepsia
Volume volume49
Issue issue3
Publisher Blackwell
Start Page 521
End Page 526
ISSN 0013-9580
NCID AA00180597
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 18031552
DOI 10.1111/j.1528-1167.2007.01411.x
Web of Science KeyUT 000253477800020
Related Url isVersionOf https://doi.org/10.1111/j.1528-1167.2007.01411.x
JaLCDOI 10.18926/AMO/31858
FullText URL fulltext.pdf
Author Ogawa, Tomoyuki| Ono, Shigeki| Ichikawa, Tomotsugu| Arimitsu, Seiji| Onoda, Keisuke| Tokunaga, Koji| Sugiu, Kenji| Tomizawa, Kazuhito| Matsui, Hideki| Date, Isao|
Abstract <p>Many studies have shown that a motif of 11 consecutive arginines (11R) is one of the most effective protein transduction domains (PTD) for introducing proteins into the cell membrane. By conjugating this &#34;11R&#34;, all sorts of proteins can effectively and harmlessly be transferred into any kind of cell. We therefore examined the transduction efficiency of 11R in cerebral arteries and obtained results showing that 11R fused enhanced green fluorescent protein (11R-EGFP) immediately and effectively penetrated all layers of the rat basilar artery (BA), especially the tunica media. This method provides a revolutionary approach to cerebral arteries and ours is the first study to demonstrate the successful transductionof a PTD fused protein into the cerebral arteries. In this review, we present an outline of our studies and other key studies related to cerebral vasospasm and 11R, problems to be overcome, and predictions regarding future use of the 11R protein transduction method for cerebral vasospasm (CV).</p>
Keywords cerebral vasospasm 11 consecutive arginines (11R) enhanced green fluorescent protein (EGFP)
Amo Type Review
Published Date 2009-02
Publication Title Acta Medica Okayama
Volume volume63
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 7
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19247417
Web of Science KeyUT 000263730300001
JaLCDOI 10.18926/AMO/32071
FullText URL fulltext.pdf
Author Takahashi, Fumio| Kuramitsu, Makoto| Tokuda, Masaaki| Matsui, Hideki| Itano, Toshifumi| Murakami, Tetsu-Hide| Hatase, Osamu| Nishida, Isamu|
Abstract <p>Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.</p>
Keywords chick growth factors cell proliferation growth regulation DNA and RNA synthesis protein synthesis
Amo Type Article
Published Date 1979-06
Publication Title Acta Medica Okayama
Volume volume33
Issue issue3
Publisher Okayama University Medical School
Start Page 167
End Page 176
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 158945
JaLCDOI 10.18926/AMO/32414
FullText URL fulltext.pdf
Author Matsui, Hideki| Kurosaki, Tomohiro| Tokuda, Masaaki| Hatase, Osamu|
Abstract <p>2-Mercaptoethanol increases the optical density of assay solutions at wavelengths between 280 to 400 nm, and therefore interferes with the measurement of protein concentration by the microbiuret method. Protein concentration can be determined in the presence of 2-mercaptoethanol up to 6 mM by modification of the method as follows: after the precipitation of protein by trichloroacetic acid in the presence of deoxycholate, the precipitate is resolubilized with NaOH solution. Dithiothreitol interfered with the protein determinations could by made in the presence of 4 mM of dithiothreitol with the modified microbiuret method. This modified method is time-saving and more reliable than other methods for protein determination, such as Lowry's method, in the presence of sulfhydryl reagents.</p>
Keywords microbiuret method sulfhydryl reagent protein determination
Amo Type Article
Published Date 1983-04
Publication Title Acta Medica Okayama
Volume volume37
Issue issue2
Publisher Okayama University Medical School
Start Page 125
End Page 129
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6869062
Web of Science KeyUT A1983QN63900004
JaLCDOI 10.18926/AMO/32903
FullText URL fulltext.pdf
Author Wu, Yumei| Tada, Mikiro| Takahata, Kyoya| Tomizawa, Kazuhito| Matsui, Hideki|
Abstract Neuronal apoptosis is involved in neurodegenerative diseases such as Alzheimer's disease and Parkinson.s disease. An efficient means of preventing it remains to be found. Some n-3 polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA, 22 : 6n-3) and eicosapentaenoic acid (EPA, 20 : 5n-3) have been reported to be protective against the neuronal apoptosis and neuronal degeneration seen after spinal cord injury (SCI) [1]. However, it is unclear which kinds of PUFAs have the most potent ability to inhibit neuronal apoptosis and whether the simultaneous treatment of PUFAs inhibits the apoptosis. In the present study, we compared the abilities of various n-3- and n-6- PUFAs to inhibit the apoptosis induced after the administration of different apoptotic inducers, etoposide, okadaic acid, and AraC, in mouse neuroblastoma cells (Neuro2a). Preincubation with DHA (22 : 6n-3), eicosapentaenoic acid (EPA, 20 : 5n-3), alpha-linolenic acid (alpha-LNA, 18 : 3n-3), linoleic acid (LA, 18 : 2n-6), arachidonic acid (AA, 20 : 4n-3), and gamma-linolenic acid (gamma-LNA, 18 : 3n-6) significantly inhibited caspase-3 activity and LDH leakage but simultaneous treatment with the PUFAs had no effect on the apoptosis of Neuro2a cells. There were no significant differences of the anti-apoptotic eff ect among the PUFAs. These results suggest that PUFAs may not be effective for inhibiting neuronal cell death after acute and chronic neurodegenerative disorders. However, dietary supplementation with PUFAs may be beneficial as a potential means to delay the onset of the diseases and/or their rate of progression.
Keywords polyunsaturated fatty acid (PUFA) neurodegenerative disease caspase neuronal apoptosis DHA
Amo Type Original Article
Published Date 2007-06
Publication Title Acta Medica Okayama
Volume volume61
Issue issue3
Publisher Okayama University Medical School
Start Page 147
End Page 152
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17593950
Web of Science KeyUT 000247574700003
FullText URL EpilepsyRes105_1_220.pdf
Author Ohmori, Iori| Hayashi, Keiichiro| Wang, Haijiao| Ouchida, Mamoru| Fujita, Naohiro| Inoue, Takushi| Michiue, Hiroyuki| Nishiki, Teiichi| Matsui, Hideki|
Published Date 2013-07
Publication Title Epilepsy Research
Volume volume105
Issue issue1-2
Publisher Elsevier Science
Start Page 220
End Page 224
ISSN 09201211
NCID AA10726642
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version author
PubMed ID 23375560
DOI 10.1016/j.eplepsyres.2013.01.003
Web of Science KeyUT 000320737500027
Related Url isVersionOf https://doi.org/10.1016/j.eplepsyres.2013.01.003
JaLCDOI 10.18926/AMO/30465
FullText URL fulltext.pdf
Author Nakamura, Mitsuo| Itano, Toshifumi| Yamaguchi, Fuminori| Mizobuchi, Masayuki| Tokuda, Masaaki| Matsui, Hideki| Etoh, Siji| Hosokawa, Kiyoshi| Ohmoto, Takashi| Hatase, Osamu|
Abstract <p>Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.</p>
Keywords protein peptide microdialysis extracellular space probe
Amo Type Article
Published Date 1990-02
Publication Title Acta Medica Okayama
Volume volume44
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 8
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2330841
Web of Science KeyUT A1990CT06800001
JaLCDOI 10.18926/AMO/30733
FullText URL fulltext.pdf
Author Noguchi, Hirofumi| Matsumoto, Shinichi| Matsushita, Masayuki| Kobayashi, Naoya| Tanaka, Koichi| Matsui, Hideki| Tanaka, Noriaki|
Abstract The development by the Edmonton group of a sirolimus-based, steroid-free, low-tacrolimus regimen is a significant breakthrough that allows the rate of insulin independence after islet transplantation to increase from 13% to 80% at 1 year ; however, the rate is reduced to 50% at 3 years, attributed to prolonged tacrolimus exposure. Recently, immunosuppression agents such as cyclosporine, mycophenolate mofetil, and the novel agent FTY 720 have been used instead of tacrolimus. Lymphocytedepleting antibodies such as anti-thymocyte globulin, alemtuzumab, and hOKT3gamma 1 (ala, ala) have been launched, and a costimulatory blockade of anti-CD40 monoclonal antibodies and CTLA4-Ig will be attempted in the near future. Moreover, the potential of a novel immunosuppressing peptide could now be realized using new technology called the protein transduction system. In this review, we show some of the most recent contributions to the advancement of knowledge in this field.
Keywords islet transplantation steroid-free Edmonton protocol protein transduction syst
Amo Type Review
Published Date 2006-04
Publication Title Acta Medica Okayama
Volume volume60
Issue issue2
Publisher Okayama University Medical School
Start Page 71
End Page 76
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16680182
Web of Science KeyUT 000237001900001
Author Kuramitsu, Makoto| Itano, Toshifumi| Matsui, Hideki| Tokuda, Masaaki| Hatase, Osamu| Murakami, Tetsuhide| Nisida, Isamu| Hayashi, Hideo|
Published Date 1979-04-30
Publication Title 岡山医学会雑誌
Volume volume91
Issue issue3-4
Content Type Journal Article
JaLCDOI 10.18926/AMO/30708
FullText URL fulltext.pdf
Author Kuramitsu, Makoto| Matsui, Hideki| Tokuda, Masaaki| Hatase, Osamu|
Abstract <p>Two factors from normal rat liver cytoplasm inhibited the proliferation of cultured L-929 fibroblasts. One was arginase, the other was a small molecular weight inhibitor stable to trypsin and heat treatment. The small molecular weight inhibitor inhibited the protein and DNA synthesis of L-cells. Inhibition of DNA synthesis was thought to be secondary to the inhibition of protein synthesis.</p>
Keywords cell proliferation growth factor inhibiting factor rat liver cytosol L-cells
Amo Type Article
Published Date 1982-02
Publication Title Acta Medica Okayama
Volume volume36
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 10
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7064729
Web of Science KeyUT A1982NE20000001
Author Fujimura, Atsushi| Michiue, Hiroyuki| Nishiki, Tei-ichi| Ohmori, Iori| Wei, Fanyan| Matsui, Hideki| Tomizawa, Kazuhito|
Published Date 2011-05-14
Publication Title PLoS ONE
Volume volume6
Issue issue3
Content Type Journal Article
JaLCDOI 10.18926/AMO/32113
FullText URL fulltext.pdf
Author Ishii, Masamitsu| Tomizawa, Kazuhito| Matsushita, Masayuki| Matsui, Hideki|
Abstract <p>The central nervous system is highly plastic and has been shown to undergo both transient and chronic adaptive changes in response to environmental influences. The purpose of this study was to investigate the effect of hypergravic field on long-term potentiation (LTP) in the mouse hippocampus. Exposure of mice to 4G fields for 48 h had no effect on input-output coupling during extracellular stimulation of Schaffer collaterals and paired pulse facilitation, suggesting that the hypergravic exposure had no detrimental effect on basal neurotransmission in the hippocampus. However, the exposure to 4G fields for 48 h significantly induced LTP compared with the control mouse hippocampus. In contrast, no significant changes of late-phase LTP (L-LTP) were found in the hippocampi of mice exposed to the hypergravic field. Exposure of mice to 4G fields for 48 h enhanced AMPA receptor phosphorylation but not cyclic AMP-responsive element binding protein (CREB) phosphorylation. These results suggest that exposure to hyperdynamic fields influences the synaptic plasticity in the hippocampus.</p>
Keywords long-term potentiation (LTP) AMPA receptor cyclic AMP-responsive element binding protein (CREB) plasticity synapse
Amo Type Article
Published Date 2004-06
Publication Title Acta Medica Okayama
Volume volume58
Issue issue3
Publisher Okayama University Medical School
Start Page 143
End Page 149
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 15471436
Web of Science KeyUT 000222273300005
JaLCDOI 10.18926/AMO/31596
FullText URL fulltext.pdf
Author Miyamoto, Osamu| Itano, Toshifumi| Fujisawa, Mutsuo| Tokuda, Masaaki| Matsui, Hideki| Nagao, Seigo| Hatase, Osamu|
Abstract <p>Basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) were administered into the rat brain following unilateral fimbria-fornix transection. Both bFGF and NGF stimulated the sprouting of acetylcholinesterase (AChE) positive fibers in the hippocampus on the lesioned side. Furthermore, a small number of AChE-positive fibers were regenerated even when only the vehicle was administered. Rats treated with NGF as well as control group had only thin fibers, whereas those treated with bFGF had not only thin fibers but also thick fibers. These results indicate that intrinsic NGF is released and acts on damaged neurons directly, while bFGF acts them on directly and/or indirectly after brain injury.</p>
Keywords bFGF NGF regeneration acetylcholinesterase positive fibers sprouting
Amo Type Article
Published Date 1993-06
Publication Title Acta Medica Okayama
Volume volume47
Issue issue3
Publisher Okayama University Medical School
Start Page 139
End Page 144
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8379341
Web of Science KeyUT A1993LL12400001