| Author | Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori| |
|---|---|
| Keywords | Biomineralization Diatom Silica Transcriptome Proteome |
| Note | This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1. </br>This fulltext is available in June 2021.| |
| Published Date | 2020-06-03 |
| Publication Title | Marine Biotechnology |
| Publisher | Springer |
| ISSN | 1436-2228 |
| NCID | AA11357643 |
| Content Type | Journal Article |
| language | フランス語 |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 32488507 |
| DOI | 10.1007/s10126-020-09976-1 |
| Web of Science KeyUT | 000537432000001 |
| Related Url | isVersionOf https://doi.org/10.1007/s10126-020-09976-1 |
| Title Alternative | Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea |
|---|---|
| FullText URL | srfa_109_001_006.pdf |
| Author | Kajiyama, Yuki| Mizobata, Satsuki| Akaji, Shusaku| Nemoto, Michiko| Tamura, Takashi| Inagaki, Kenji| |
| Abstract | Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity. |
| Abstract Alternative | , , , |
| Keywords | glycine oxidase Marinomonas mediterranea cysteine tryptophilquinone recombinant expression enzymatic glycine assay |
| Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
| Published Date | 2020-02-01 |
| Volume | volume109 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 2186-7755 |
| language | 日本語 |
| File Version | publisher |
| Author | Tamura, T.| Ibi, T.| Inagaki, K.| Kubo, Y.| Okuda, K.| |
|---|---|
| Published Date | 2016-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume105 |
| Content Type | Departmental Bulletin Paper |
| Author | Tamura, Takashi| Tsunekawa, Naoki| Nemoto, Michiko| Inagaki, Kenji| Hirano, Toshiyuki| Sato, Fumitoshi| |
|---|---|
| Published Date | 2016-01-28 |
| Publication Title | Scientific reports |
| Volume | volume6 |
| Content Type | Journal Article |
| Author | Oshima, Kenshiro| Hattori, Masahira| Shimizu, Hitomi| Fukuda, Koji| Nemoto, Michiko| Inagaki, Kenji| Tamura, Takashi| |
|---|---|
| Published Date | 2015-07-09 |
| Publication Title | Genome Announcements |
| Volume | volume3 |
| Issue | issue4 |
| Content Type | Journal Article |
| Author | Nakai, Ryuichiro| Fujino, Shihoko| Utsumi, Tomohiro| Tamura, Takashi| Kusakabea, Hitoshi| Inagaki, Kenji| |
|---|---|
| Published Date | 2014-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume103 |
| Content Type | Departmental Bulletin Paper |
| Author | Kobayashi, Fumiaki| Aomine, Hiroki| Mizunashi, Wataru| Yu, Fujio| Tamura, Takashi| Inagaki, Kenji| |
|---|---|
| Published Date | 2012-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume101 |
| Content Type | Departmental Bulletin Paper |
| Author | Maemura, Tomomi| Uchitomi, Kumiko| Kusaka, Chika| Inagaki, Junko| Tamura, Takashi| Soda, Kenji| Inagaki, Kenji| |
|---|---|
| Published Date | 2011-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume100 |
| Content Type | Departmental Bulletin Paper |
| Author | Takahata, Muneaki| Tamura, Takashi| Abe, Katsumasa| Mihara, Hisaaki| Kurokawa, Suguru| Yamamoto, Yoshihiro| Nakano, Ryuhei| Esaki, Nobuyoshi| Inagaki, Kenji| |
|---|---|
| Published Date | 2008-06-26 |
| Publication Title | Journal of Biochemistry |
| Volume | volume143 |
| Issue | issue4 |
| Content Type | Journal Article |
| Author | Shinozaki, Mai| Yanagitani, Masahiko| Kaneda, Shouichirou| Kudou, Daizou| Endou, Yuuichi| Tamura, Takashi| Kuramitsu, Seiki| Inagaki, Kenji| |
|---|---|
| Published Date | 2010-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume99 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Fukuda, Koji| Tamura, Takashi| Inagaki, Kenji| |
|---|---|
| Published Date | 2010-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume99 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Yanagitani, Masahiko| Uemae, Satoshi| Shiraga, Tomoyuki| Tamura, Takashi| Inagaki, Kenji| |
|---|---|
| Published Date | 2009-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume98 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Tachibana, Akiko| Tamura, Takashi| Imada, Katsumi| Kinoshita, Miki| Namba, Keiichi| Tsutsumi, Noriko| Hashida, Miyoko| Sakaguchi, Hiromichi| Inagaki, Kenji| |
|---|---|
| Published Date | 2008-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume97 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Tamura, Takashi| |
|---|---|
| Published Date | 1995-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume84 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Seow, Teck Keong| Inagaki, Kenji| Tamura, Takashi| Tanaka, Hidehiko| |
|---|---|
| Published Date | 1997-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume86 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Inoue, Hiroyuki| Tamura, Takashi| Inagaki, Kenji| Tanaka, Hidehiko| |
|---|---|
| Published Date | 1998-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume87 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Inagaki, Kenji| Kawaguchi, Masakazu| Taguchi, Takaaki| Tamura, Takashi| Tanaka, Hidehiko| |
|---|---|
| Published Date | 1998-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume87 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Arima, Jiro| Tamura, Takashi| Shinohara, Hiroaki| Kusakabe, Hitoshi| Tanaka, Hidehiko| Inagaki, Kenji| |
|---|---|
| Published Date | 2002-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume91 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Takahashi, Tatsuya| Tanaka, Noboru| Tamura, Takashi| Mukai, Chika| Isobe, Kimiyasu| Wakao, Norio| Tanaka, Hidehiko| Inagaki, Kenji| |
|---|---|
| Published Date | 2002-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume91 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Tamura, Takashi| Li, Yin Shu| Tanaka, Hidehiko| Inagaki, Kenji| |
|---|---|
| Published Date | 2002-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume91 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
