FullText URL Biochem_Biophy_Res_Comm_201807.pdf
Author Fujiwara, Toshifumi| Eguchi, Takanori| Sogawa, Chiharu| Ono, Kisho| Murakami, Jun| Ibaragi, Soichiro| Asaumi, Jun-ichi| Okamoto, Kuniaki| Calderwood, Stuart K.| Kozaki, Ken-ichi|
Abstract Genetic amplification, overexpression, and increased signaling from the epidermal growth factor receptor (EGFR) are often found in oral squamous cell carcinoma (OSCC) and thus EGFR is frequently targeted molecularly by the therapeutic antibody cetuximab. We assessed effects of cetuximab in control of EGF-driven malignant traits of OSCC cells. EGF stimulation promoted progression level of mesenchymal traits in OSCC cells, which were attenuated by cetuximab but incompletely. We pursued a potential mechanism underlying such incomplete attenuation of OSCC malignant traits. Cetuximab promoted secretion of EGFR-EVs by OSCC cells and failed to inhibit EGF-driven secretion of EGFR-EVs. Cetuximab was also found to be robustly secreted with the EGFR-EVs by the OSCC cells. Thus, EGF promotes the level of mesenchymal traits of OSCC cells and secretion of EGFR-EVs, which involve cetuximab resistance.
Keywords Extracellular vesicles Anti-EGFR antibody therapy Cetuximab Epithelial-to-mesenchymal transition Head and neck squamous cell carcinoma
Published Date 2018-07-13
Publication Title Biochemical and Biophysical Research Communications A
Publisher Elsevier
ISSN 0006291X
NCID AA00564395
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders http://creativecommons.org/licenses/by-nc-nd/4.0/
File Version publisher
DOI 10.1016/j.bbrc.2018.07.035
Related Url isVersionOf https://doi.org/10.1016/j.bbrc.2018.07.035