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ID 11785
Eprint ID
11785
FullText URL
Thumnail K003330.pdf 87.5 KB
Title Alternative
ヒト初乳由来分泌型IgAはボツリヌスB型16S毒素と結合し,毒素のT84細胞への結合を抑制する
Author
Matsumura, Takuhiro
Abstract
Botulinum neurotoxin produced by Clostridium botulinum type B is in the form of a complex of 12S and 16S toxins. Food-borne botulism is caused by these complex toxins which are ingested orally and absorbed from the digestive tract. Here, we show that the human milk SIgA binds to the type B16S toxin. The binding of SIgA to 16S toxin and HA was inhibited by carbohydrates such as galactose, suggesting that the interaction of carbohydrate side chain of the SIgA with the HA of the 16S toxin is important for SIgA-16S complex formation. We also demonstrate that SIgA inhibits the attachment of 16S toxin to intestinal epithelial cells. These data suggest that the interaction of antigen nonspecific SIgA with 16S toxin has a large influence on the absorption of 16S toxin from the intestinal epithelium, and that SIgA may provide insight into developing a therapeutic agent for type B food-borne botulism.
Keywords
Clostridium
Botulinum
Botulism
Hemagglutinin
Neurotoxin
Toxin
Secretory IgA
Immunoglobulin
Galactose
Innate immunity
Note
http://dx.doi.org/10.1016/j.bbrc.2006.11.095
Published Date
2007-03-23
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3330号
Granted Date
2007-03-23
Thesis Type
Doctor of Philosophy in Medical Science
Grantor
岡山大学
Official Url
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17156748&dopt=Abstract
language
日本語
File Version
none
Refereed
Unknown