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ID 11745
Eprint ID
11745
FullText URL
Thumnail K003321.pdf 85.5 KB
Title Alternative
EGF+FGF2髄腔内投与によるALSモデルマウス脊髄での神経幹細胞増殖効果
Author
Ohta, Yasuyuki
Abstract
We investigated three steps of neural precursor cell activation--proliferation, migration, and differentiation--in amyotrophic lateral sclerosis spinal cord treated with intrathecal infusion of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2) into the lumbar spinal cord region of normal and symptomatic transgenic (Tg) mice with a mutant human Cu/Zn superoxide dismutase (SOD1) gene. We observed that 5-bromodeoxyuridine (BrdU) + nestin double-labeled neural precursor cells increased in the spinal cords of Tg mice compared with non-Tg mice, with a much greater increase produced by EGF and FGF2 treatment. The number of BrdU + nestin double-labeled cells was larger than that of BrdU + ionized calcium-binding adapter molecule-1 (Iba1), BrdU + glial fibrillary acidic protein (GFAP), or BrdU + highly polysialylated neural cell adhesion molecule (PSA-NCAM) double-labeled cells, but none expressed neuronal nuclear antigen (NeuN). On further analysis of the gray matter of Tg mice, the number of BrdU + nestin and BrdU + PSA-NCAM double-labeled cells increased more in the ventral horns than the dorsal horns, which was again greatly enhanced by EGF and FGF2 treatment. Because neural precursor cells reside close to the ependyma of central canal, the present study suggests that proliferation and migration of neural precursor cells to the ventral horns is greatly activated in symptomatic Tg mice and is further enhanced by EGF and FGF2 treatment and, furthermore, that the neural precursor cells preferentially differentiate into neuronal precursor cells instead of astrocytes in Tg mice with EGF and FGF2 treatment.
Keywords
neural precursor cells
epidermal growth factor
fibroblast growth factor 2
spinal cord
amyotrophic lateral sclerosis
Note
http://dx.doi.org/10.1002/jnr.21017
Published Date
2007-03-23
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3321号
Granted Date
2007-03-23
Thesis Type
博士(医学)
Grantor
岡山大学
language
日本語
File Version
none
Refereed
Unknown