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ID 11744
Eprint ID
11744
FullText URL
Thumnail K003320.pdf 107 KB
Title Alternative
多価不飽和脂肪酸の神経芽腫細胞(Neuro2a)におけるアポトーシス抑制効果
Author
Wu, Yumei
Abstract
Neuronal apoptosis is involved in neurodegenerative diseases such as Alzheimer's disease and Parkinson.s disease. An efficient means of preventing it remains to be found. Some n-3 polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA, 22 : 6n-3) and eicosapentaenoic acid (EPA, 20 : 5n-3) have been reported to be protective against the neuronal apoptosis and neuronal degeneration seen after spinal cord injury (SCI) [1]. However, it is unclear which kinds of PUFAs have the most potent ability to inhibit neuronal apoptosis and whether the simultaneous treatment of PUFAs inhibits the apoptosis. In the present study, we compared the abilities of various n-3- and n-6- PUFAs to inhibit the apoptosis induced after the administration of different apoptotic inducers, etoposide, okadaic acid, and AraC, in mouse neuroblastoma cells (Neuro2a). Preincubation with DHA (22 : 6n-3), eicosapentaenoic acid (EPA, 20 : 5n-3), alpha-linolenic acid (alpha-LNA, 18 : 3n-3), linoleic acid (LA, 18 : 2n-6), arachidonic acid (AA, 20 : 4n-3), and gamma-linolenic acid (gamma-LNA, 18 : 3n-6) significantly inhibited caspase-3 activity and LDH leakage but simultaneous treatment with the PUFAs had no effect on the apoptosis of Neuro2a cells. There were no significant differences of the anti-apoptotic eff ect among the PUFAs. These results suggest that PUFAs may not be effective for inhibiting neuronal cell death after acute and chronic neurodegenerative disorders. However, dietary supplementation with PUFAs may be beneficial as a potential means to delay the onset of the diseases and/or their rate of progression.
Keywords
polyunsaturated fatty acid (PUFA)
neurodegenerative disease
caspase
neuronal apoptosis
DHA
Note
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17593950&dopt=Abstract
Published Date
2007-03-23
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3320号
Granted Date
2007-03-23
Thesis Type
博士(医学)
Grantor
岡山大学
Official Url
http://escholarship.lib.okayama-u.ac.jp/cgi/viewcontent.cgi?article=2781&context=amo
language
日本語
File Version
none
Refereed
Unknown