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ID 11678
Eprint ID
11678
FullText URL
Thumnail K003302.pdf 83.3 KB
Title Alternative
11Rを用いた新しいタンパク質導入法―脳血管障害を治療するための効果的な新しい薬物伝達システム―
Author
Ogawa, Tomoyuki
Abstract
BACKGROUND AND PURPOSE: A motif of 11 consecutive arginines (11R) is reported to be one of the most effective protein transduction domains for introducing proteins into the cell membrane. We therefore examined the transduction efficiency of 11R in cerebral arteries. METHODS: Basilar arteries (BAs) obtained from rats were incubated with either 11R-enhanced green fluorescent protein (11R-EGFP) or EGFP without 11R. After incubation, expression of 11R-EGFP or EGFP in BA serial sections was observed by fluorescence microscope. In an additional in vivo experiment, 11R-EGFP or EGFP was injected into the cisterna magna with or without subarachnoid hemorrhage. The 11R-EGFP or EGFP was injected just after the autologous blood injection, and then the expression of 11R-EGFP or EGFP in BA sections was also observed by fluorescence microscope. RESULTS: The 11R-EGFP signal was much stronger than that of EGFP in all layers of the rat BA, in both in vivo and ex vivo experiments. Moreover, the 11R-EGFP was transduced into the BA immediately (2 hours after the injection). Interestingly, 11R-fused fluorescent protein was transduced especially into the tunica media of the BA. CONCLUSIONS: The 11R-fused fluorescent protein effectively penetrates into all layers of the rat BA, especially into the tunica media. This is the first study to our knowledge to demonstrate the successful transduction of a protein transduction domain fused protein into the cerebral arteries.
Note
http://dx.doi.org/10.1161/01.STR.0000259887.70358.e0
Published Date
2007-03-23
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3302号
Granted Date
2007-03-23
Thesis Type
Doctor of Philosophy in Medical Science
Grantor
岡山大学
Official Url
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17332457&dopt=Abstract
language
日本語
File Version
none
Refereed
Unknown