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ID 8515
Eprint ID
8515
FullText URL
Thumnail K002988.pdf 49.3 KB
Title Alternative
Des-gamma-carboxy Prothrombinは肝細胞癌のAutologousな増殖因子である
Author
Suzuki, Mayumi
Abstract
Des-gamma-carboxyl prothrombin (DCP) is a well recognized tumor marker for hepatocellular carcinoma (HCC). In the present study, we demonstrate that DCP has a mitogenic effect on HCC cell lines. Purified DCP stimulated DNA synthesis of Hep3B and SK-Hep-1 cells in a dose-dependent manner. DCP was found to bind with cell surface receptor Met causing Met autophosphorylation and also to activate STAT3 signaling pathway through Janus kinase 1. Luciferase gene reporter analysis showed that DCP induced STAT3-related transcription. Small interfering RNAs against both STAT3 and Met abrogated DCP-induced cell proliferation. DCP did not affect the mitogen-activated protein kinase pathway, Myc signaling pathway, or phosphoinositide 3-kinase/Akt pathway. Based on these results, we believe that DCP acts as an autologous mitogen for HCC cell lines. The Met-Janus kinase 1-STAT3 signaling pathway may be a major signaling pathway for DCP-induced cell proliferation.
Note
http://dx.doi.org/10.1074/jbc.M406714200
Published Date
2005-06-30
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第2988号
Granted Date
2005-06-30
Thesis Type
博士(医学)
Grantor
岡山大学
Official Url
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15582995&dopt=Abstract
language
日本語
File Version
none
Refereed
Unknown