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ID 4688
Eprint ID
4688
FullText URL
Thumnail K002093.pdf 173 KB
Title Alternative
ラット海馬の錐体細胞におけるDNAトポイソメラーゼⅡβ発現の領域による違い
Author
Abstract
A detailed analysis of the differential expression of a nuclear enzyme, DNA topoisomerase (topo) IIβ, was performed in the rat hippocampal pyramidal layer. Three-dimensional (3-D) reconstruction from serial sections immunostained with anti-topo IIβ antibody showed that the immunoreactivity was apparently weak in the entire CA3 region. Almost all CA1 pyramidal cells showed similar immunoreactivity to that seen in the dentate granular cells, the subicular neurons, and the cerebral neocortical neurons. In addition, immunoblotting analysis in the adult dorsal hippocampus showed that the expression level of topo IIβ in the CA3 was significantly lower than that in the CA1 region. The dissociation in the expression level between CA1 and CA3 occurred in postnatal days 4 (P4) through P6. The present finding suggests that the enzyme is possibly involved in activities of the hippocampal pyramidal neurons.
Keywords
DNA topoisomerase IIβ
Hippocampal pyramidal cells
Immunohistochemistry
3-D reconstruction
Western blotting
Postnatal development
Note
掲載順位 77 http://dx.doi.org/10.1016/S0168-0102(00)00098-5
Published Date
2000-03-31
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第2093号
Granted Date
2000-03-31
Thesis Type
博士(医学)
Grantor
岡山大学
Official Url
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10771107&dopt=Abstract
language
日本語
File Version
none
Refereed
Unknown