JaLCDOI 10.18926/AMO/30413
FullText URL fulltext.pdf
Author Zhao, Yuan-Qing| Kinuta, Masahiro| Abe, Tadashi| Yao, Wen-Bin| Ubuka, Toshihiko|
Abstract <p>The effects of intraperitoneal administration of 2-(4-carboxy-D-gluco-tetrahydroxybutyl)thiazolidine-4-carboxylic acid (CGUA), a cysteine derivative conjugated with glucuronic acid, on total glutathione and total cysteine contents in rat tissues were investigated. Total glutathione (GSH and GSSG) and total cysteine (cysteine and cystine) were determined by a new method consisting of preparation of S-carboxymethylglutathione (CMSG) and S-carboxymethylcysteine (CMC), respectively, and subsequent analyses with an amino acid analyzer. CGUA was determined by a coloration method employing an acidic ninhydrin reagent. Total cysteine contents in liver, kidney and plasma rapidly increased to 2.3, 2.7 and 6.5 times the levels of the controls, respectively, after CGUA administration at a dose of 5 mmol/kg of body weight. Total glutathione content did not change significantly in the liver or blood except for the kidney with a significant increase during the first 1-h period after administration. CGUA content increased markedly in these tissues, especially in the kidney, and 30% of administered CGUA was excreted in urine within 2h. These results indicate that CGUA is converted into cysteine in vivo, suggesting the usefulness of this compound for protection of the kidney and the liver.</p>
Keywords cysteine glutathione S-carboxymethylglutathione S-carboxymethylcysteine cysteineglucuronic acied
Amo Type Article
Published Date 1995-02
Publication Title Acta Medica Okayama
Volume volume49
Issue issue1
Publisher Okayama University Medical School
Start Page 35
End Page 42
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7762408
Web of Sience KeyUT A1995QK32500006