start-ver=1.4
cd-journal=joma
no-vol=54
cd-vols=
no-issue=5
article-no=
start-page=185
end-page=192
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2000
dt-pub=200010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Diagnostic value of the detection of t(14;18) chromosome translocation in malignant hematological and immunopathological diseases using polymerase chain reaction.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The majority of the t(14;18) chromosome translocations that occur in non-Hodgkin centroblastic-centrocytic follicular lymphoma can be detected by various methods. During the translocation process the bcl-2 gene located on chromosome 18 (18q21) is translocated to the JH region of the immunoglobulin gene of chromosome 14 (14q32). The most frequent type of bcl-2 translocations is the mbr type, whereas the immunoglobulin gene breaks mainly at the JH1-6 exons. About one of the 10(5) cells bearing the translocation can already be detected by using nested polymerase chain reaction (PCR). Eight patients suffering from follicular lymphoma were included in this study, which considered the usefulness of the PCR method. The results are in good agreement with those obtained by conventional diagnostic methods. Translocation can be detected, however, in patients with non-malignant diseases such as Sjogren's syndrome (about 5% of the patients) and in a patient with Whipple disease. In addition, translocation was detected in lymphocytes of peripheral blood of a healthy donor. Since lymphomas are detected in patients with Sjogren's syndrome with a relative high frequency, an early diagnosis of the translocation could improve the treatment of the disease. Nevertheless, a diagnosis of lymphoma is valid only in cases of bone marrow translocation-positivity.</p>

en-copyright=
kn-copyright=

en-aut-name=TakacsIstvan
en-aut-sei=Takacs
en-aut-mei=Istvan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ZeherMargit
en-aut-sei=Zeher
en-aut-mei=Margit
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UrbanLaszio
en-aut-sei=Urban
en-aut-mei=Laszio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SzegediGyula
en-aut-sei=Szegedi
en-aut-mei=Gyula
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SemseiImre
en-aut-sei=Semsei
en-aut-mei=Imre
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=University of Debrecen

affil-num=2
en-affil=
kn-affil=University of Debrecen

affil-num=3
en-affil=
kn-affil=University of Debrecen

affil-num=4
en-affil=
kn-affil=University of Debrecen

affil-num=5
en-affil=
kn-affil=University of Debrecen
en-keyword=t(14;18) chromosome translocation
kn-keyword=t(14;18) chromosome translocation
en-keyword=lymphoma
kn-keyword=lymphoma
en-keyword=early diagnoses
kn-keyword=early diagnoses
en-keyword=Sjogren's syndrome
kn-keyword=Sjogren's syndrome
END