このエントリーをはてなブックマークに追加
ID 32848
JaLCDOI
FullText URL
Thumnail fulltext.pdf 1.54 MB
Author
Huang, Zhenyu
Liu, Qingping
Li, Wenzhe
Wang, Renjun
Wang, Dan
Zhang, Yingbiao
Zhang, Fan
Chi, Yan
Liu, Zhe
Liu, Zibo
Zhang, Qiming
Abstract

We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.

Keywords
7-KC
NF-?B
apoptosis
atherosclerosis
Amo Type
Original Article
Published Date
2010-04
Publication Title
Acta Medica Okayama
Volume
volume64
Issue
issue2
Publisher
Okayama University Medical School
Start Page
85
End Page
93
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT