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ID 48280
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Author
Khan, Shahbaz Manzoor
Debnath, Chanchal
Pramanik, Amiya Kumar
Xiao, Lihua
Nozaki, Tomoyoshi
Ganguly, Sandipan
Abstract
Few studies in the past have examined the genetic diversity and zoonotic potential of Cryptosporidium in dairy cattle in India. To assess the importance of these animals as a source of human Cryptosporidium infections, fecal samples from 180 calves, heifers and adults and 51 farm workers on two dairy farms in West Bengal, India were genotyped by PCR-RFLP analysis of the 18S rRNA gene of Cryptosporidium followed by DNA sequencing of the PCR products. Phylogenetic analysis was carried out on the DNA sequences obtained in the study and those available in GenBank. The overall prevalence of Cryptosporidium in cattle was 11.7% though the infection was more prevalent in younger calves than in adult cattle. The occurrence of Cryptosporidium parvum, Cryptosporidium bovis, Cryptosporidium ryanae and Cryptosporidium andersoni in cattle followed an age-related pattern. A Cryptosporidium suis-like genotype was also detected in a calf. Farm workers were infected with Cryptosporidium hominis, C. parvum and a novel C. bovis genotype. These findings clearly suggest that there is a potential risk of zoonotic transmission of Cryptosporidium infections between cattle and humans on dairy farms in India.
Keywords
Cryptosporidium
Dairy cattle
Zoonoses
India
Genotyping
Phylogenetic analysis
Published Date
2010-07-15
Publication Title
Veterinary Parasitology
Volume
volume171
Issue
issue1-2
Publisher
Elsevier
Start Page
41
End Page
47
ISSN
0304-4017
NCID
AA00883655
Content Type
Journal Article
Project
Collaborative Research of Okayama University for Infectious Diseases in India
Official Url
http://www.sciencedirect.com/science/article/pii/S0304401710001652
language
英語
Copyright Holders
© 2010 Elsevier B.V. All rights reserved.
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Refereed
True
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