JaLCDOI 10.18926/AMO/32446
FullText URL fulltext.pdf
Author Wahid, Syarifuddin|
Abstract <p>Primary cultures of liver cells from normal adult rats were treated with 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) at various concentrations for 6 days. 3'-Me-DAB treatment induced rapid proliferation of epithelial clear cells with chromosomal abnormalities and gamma-glutamyl transpeptidase (GGT) activity. In early culture, marker chromosomes were detected in 13 of 44 3'-Me-DAB-treated cultures but not in control cultures. GGT activity was not detected in the epithelial clear cells in either 3'-Me-DAB-treated or control cultures. In late culture, 21 cell lines established from 39 carcinogen-treated cultures consisted of 3 diploid cell lines, 5 pseudodiploid cell lines and 13 aneuploid cell lines. Eighteen of these 21 cell lines had marker chromosomes. Of the 2 cell lines established from 15 control cultures both were aneuploid, but a marker chromosome was detected in only one of these. GGT activity was detected in 11 of 21 cell lines established from the carcinogen-treated cultures but not in those from control cultures. Morphological features of the cell lines which varied from normal to cancerous included polymorphism, increased nuclear/cytoplasmic ratio and prominent nucleoli. No cell line established in this study developed tumors in host rats during a 1-year observation period.</p>
Keywords primary liver cell cultures 3'-methyl-4-dimethyl-aminoazobenzene proliferation of epithelial clear cells chromosomal abnormality gamma- glutamyl transpeptidase
Amo Type Article
Published Date 1983-02
Publication Title Acta Medica Okayama
Volume volume37
Issue issue1
Publisher Okayama University Medical School
Start Page 31
End Page 44
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6133413
Web of Sience KeyUT A1983QD83600004
JaLCDOI 10.18926/AMO/31263
FullText URL fulltext.pdf
Author Miyazaki, Masahiro| Matsuura, Kazuhiko| Wahid, Syarifuddin| Izumi, Masaki| Taketa, Kazuhisa| Sato, Jiro|
Abstract <p>Alpha-getoprotein (AFP) was purified from fetal rat serum by a combined technique of affinity chromatography with Affi-Gel Blue and disc electrophoresis followed by extraction of AFP from the gel. The purified AFP was immunologically identical with the original AFP in fetal rat serum.</p>
Keywords purification of rat AFP fetal rat serum affinity chromatography disc electrophoresis.
Amo Type Brief Note
Published Date 1981-12
Publication Title Acta Medica Okayama
Volume volume35
Issue issue6
Publisher Okayama University Medical School
Start Page 427
End Page 430
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6172956
Web of Sience KeyUT A1981MV25300007
JaLCDOI 10.18926/AMO/30360
FullText URL fulltext.pdf
Author Wahid, Syarifuddin| Miyazaki, Masahiro| Sato, Jiro|
Abstract <p>The dispase perfusion technique was used to isolate liver cells from adult rats. The optimum conditions for obtaining many isolated liver cells with high viability were an enzyme concentration of 2000 U/ml, a pH of 7.5 and a perfusion time of 20 min. The population of isolated liver cells prepared with dispase consisted of 43.6% cells with diameters less than 20 micron and 56.4% cells with diameters above 20 micron. The isolated liver cells were cultured in basal culture medium either supplemented with or without dexamethasone (1 X 10(-5)M) and insulin (10 micrograms/ml). The addition of hormones to the culture medium improved the attachment efficiency of the isolated liver cells and delayed the disappearance of mature hepatocytes. Epithelial-like clear cells proliferated early in primary culture even in the presence of hormones. Therefore, functioning mature hepatocytes and proliferating epithelial-like clear cells coexisted well in the hormone-containing medium. Furthermore, the number of cultured cells reached a maximal level earlier in the presence of hormones than in the absence of hormones. The level of TAT activity in primary cultured cells was higher up to 3 days after inoculation in the presence of hormones than in their absence. No difference between G6Pase activity in primary cultured cells in the presence of hormones and that in the absence of hormones was found.</p>
Keywords dispase-liver-perfusion sizu distribution primary liver cell culture grouwth pattern liver-specific functions
Amo Type Article
Published Date 1984-06
Publication Title Acta Medica Okayama
Volume volume38
Issue issue3
Publisher Okayama University Medical School
Start Page 251
End Page 260
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6147068
Web of Sience KeyUT A1984SY25800005